Ashby P, Robinson D S
Biochem J. 1980 Apr 15;188(1):185-92. doi: 10.1042/bj1880185.
The lipoprotein lipase activity of epididymal fat-bodies from starved rats was measured during incubations at 37 degrees C in vitro. Protein synthesis independent activation of the enzyme, previously observed during incubations at 25 decrease C, also occurs at 37 degrees C. Protein-synthesis-dependent increases in the activity of the enzyme occur in the presence of insulin and are markedly potentiated by glucocorticoids. The effects on the activity of the enzyme of insulin alone, or in the presence of glucocorticoids, are correlated with its effects on total protein synthesis in the tissue. Adrenaline antagonizes the increase in activity of the enzyme brought about by insulin and abolishes the potentiation of insulin action by glucocorticoids. These changes may be due, at least in part, to its stimulation of inactivation of the enzyme in the tissue. It is suggested that changes in adipose-tissue lipoprotein lipase activity that occur with changes in nutritional status in vivo result from the combined effects of changes in plasma insulin and glucocorticoid concentrations.
在体外37℃孵育期间，测定了饥饿大鼠附睾脂肪体的脂蛋白脂肪酶活性。先前在25℃孵育期间观察到的该酶的蛋白质合成非依赖性激活，在37℃时也会发生。在胰岛素存在的情况下，该酶活性会出现蛋白质合成依赖性增加，并且糖皮质激素会显著增强这种增加。单独胰岛素或在糖皮质激素存在下对该酶活性的影响，与其对组织中总蛋白质合成的影响相关。肾上腺素会拮抗胰岛素引起的酶活性增加，并消除糖皮质激素对胰岛素作用的增强作用。这些变化可能至少部分是由于其刺激了组织中该酶的失活。有人提出，体内营养状况变化时脂肪组织脂蛋白脂肪酶活性的变化是血浆胰岛素和糖皮质激素浓度变化共同作用的结果。
