脂肪组织中脂蛋白脂肪酶活性的翻译后调控
Post-translational regulation of lipoprotein lipase activity in adipose tissue.
作者信息
Ashby P, Bennett D P, Spencer I M, Robinson D S
出版信息
Biochem J. 1978 Dec 15;176(3):865-72. doi: 10.1042/bj1760865.
Abstract
Changes in adipose-tissue lipoprotein lipase activity that are independent of protein synthesis were investigated in an incubation system in vitro. Under appropriate conditions at 25 degrees C a progressive increase in the enzyme activity occurs that is energy-dependent. Part of the enzyme is rapidly inactivated when the tissue is incubated with adrenaline or adrenaline plus theophylline. The mechanism of this inactivation appears to be distinct from, and to follow, the activation of the enzyme. A hypothesis is presented to account for the results in terms of an activation of the enzyme during obligatory post-translational processing and a catecholamine-regulated inactivation of the enzyme as an alternative to secretion from the adipocyte.
摘要
在体外孵育系统中研究了脂肪组织脂蛋白脂肪酶活性与蛋白质合成无关的变化。在25℃的适宜条件下，酶活性会出现能量依赖性的逐渐增加。当组织与肾上腺素或肾上腺素加茶碱一起孵育时，部分酶会迅速失活。这种失活机制似乎与酶的激活不同，且发生在激活之后。本文提出了一个假说，根据在翻译后加工过程中酶的激活以及儿茶酚胺调节的酶失活（作为脂肪细胞分泌的替代方式）来解释这些结果。
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