Koeffler H P, Golde D W
Blood. 1980 Sep;56(3):344-50.
Several human acute myeloid leukemia cell lines were recently established. These lines provide model systems to study the control of differentiation in human myelogenous leukemia and, in a broader framework, the controls of normal myeloid development. The K562 line is composed of undifferentiated blast cells that are rich in glycophorin and may be induced to produce fetal and embryonic hemoglobin in the presence of hemin. The KG-1 cell line is composed predominantly of myeloblasts and promyelocytes. A unique characteristic of the KG-1 cells is their almost complete dependence on colony-stimulating factor for proliferation in soft-gel culture. The HL-60 is a promyelocytic leukemia cell line. In the presence of DMSO, the cells mature into granulocytes. Both the KG-1 and HL-60 cells differentiate into nondividing mononuclear phagocytes when exposed to phorbol esters. Investigations with these cell lines, and selected variants should provide important insights into the cell biology and perhaps therapy of human leukemia.
最近建立了几种人类急性髓系白血病细胞系。这些细胞系提供了模型系统，用于研究人类髓性白血病中分化的控制，以及在更广泛的框架内研究正常髓系发育的控制。K562细胞系由富含血型糖蛋白的未分化原始细胞组成，在有氯化血红素存在的情况下可被诱导产生胎儿和胚胎血红蛋白。KG-1细胞系主要由成髓细胞和早幼粒细胞组成。KG-1细胞的一个独特特征是它们在软凝胶培养中增殖几乎完全依赖集落刺激因子。HL-60是一种早幼粒细胞白血病细胞系。在二甲基亚砜存在的情况下，这些细胞成熟为粒细胞。当暴露于佛波酯时，KG-1和HL-60细胞都会分化为不分裂的单核吞噬细胞。对这些细胞系以及选定变体的研究应该能为人类白血病的细胞生物学乃至治疗提供重要的见解。
