Chromatographic purification and homogeneity of extracellular acid proteinase of Aspergillus Fumigatus.

The extracellular acid proteinase from A. fumigatus was purified by employing several chromatographic procedures and a 164 fold purification with 22% yield was achieved. The homogeneity of the enzyme was confirmed by SDS-Polyacrylamide gel electrophoresis, immodiffusion and immunoelectrophoresis techniques.