一种采用51Cr标记巨噬细胞的单向迁移抑制体外测定法。
An in vitro assay for uni-directional migration inhibition employing 51Cr-labelled macrophages.
作者信息
Noronha-Blob L, Huang S W
出版信息
Clin Exp Immunol. 1980 Jun;40(3):627-32.
An improved in vitro technique to assay for migration inhibitory factor is presented. The method employs chromium-51-radiolabelled guinea-pig macrophages and offers significant advantages including (1) elimination of observer to observer variation and tedious measurements resulting in an objective and technically simple assay, (2) the requirement for small numbers of immune lymphocytes, (3) good sensitivity and reproducibility between successive assays performed on different days, and (4) a means of obtaining relative estimates of the 'strength' (concentration) of the factor so that comparisons with healthy individuals can be made.
本文介绍了一种用于检测迁移抑制因子的改进体外技术。该方法采用铬-51放射性标记的豚鼠巨噬细胞，具有显著优势，包括：（1）消除了观察者之间的差异以及繁琐的测量，从而实现了客观且技术简单的检测；（2）对免疫淋巴细胞数量需求少；（3）在不同日期进行的连续检测之间具有良好的敏感性和可重复性；（4）能够获得该因子“强度”（浓度）的相对估计值，以便与健康个体进行比较。
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