Rocklin R E
J Immunol. 1976 Mar;116(3):816-20.
The role of monosaccharides on the interaction of two lymphocyte mediators related to the human cell migration inhibition systems with their respective target cells has been investigated. Human MIF activity on blood monocytes was significantly reduced by several 5-methylpentose sugars indcluding L-fucose, L-rhamnose and 6 deoxy-D-glucose. Certain structurally unrelated monosaccharides such as D-galactose, methyl alpha-D-mannoside and N-acetyl-D-glucosamine had no effect on this system. Incubation of human monocytes with alpha-L-fucosidase, a glycosidase which cleaves terminal L-fucose moieties, reversibly prevented their response to MIF. These results indicate that alpha-L-fucosyl residues form an integral part of the receptor for MIF on human monocytes, and furthermore, this interaction may be somewhat nonspecific in that the 5-methylpentose configuration perse is recognized. In contrast, human LIF activity on PMN leukocytes was significantly reduced by N-acetyl-D-galactosamine, but not by 5-methylpentoses, D-glucose, N-acetyl-D-galactosamine, methyl alpha-Dmannoside or D-galactose. The latter finding suggests that a subterminal sugar may form part of the receptor to LIF on human PMN leukocytes.
已经研究了单糖在两种与人类细胞迁移抑制系统相关的淋巴细胞介质与其各自靶细胞相互作用中的作用。几种5-甲基戊糖，包括L-岩藻糖、L-鼠李糖和6-脱氧-D-葡萄糖，可显著降低人血单核细胞上的人巨噬细胞移动抑制因子（MIF）活性。某些结构不相关的单糖，如D-半乳糖、α-D-甲基甘露糖苷和N-乙酰-D-葡萄糖胺，对该系统没有影响。用α-L-岩藻糖苷酶（一种可切割末端L-岩藻糖部分的糖苷酶）孵育人单核细胞，可可逆地阻止它们对MIF的反应。这些结果表明，α-L-岩藻糖基残基是人类单核细胞上MIF受体的组成部分，此外，这种相互作用可能有点非特异性，因为5-甲基戊糖构型本身就被识别。相比之下，N-乙酰-D-半乳糖胺可显著降低人多形核白细胞（PMN）上的人淋巴细胞抑制因子（LIF）活性，但5-甲基戊糖、D-葡萄糖、N-乙酰-D-半乳糖胺、α-D-甲基甘露糖苷或D-半乳糖则没有这种作用。后一发现表明，一个亚末端糖可能是人类PMN白细胞上LIF受体的一部分。
