Heller A H, Spector R, Aalyson M
J Antibiot (Tokyo). 1980 Jun;33(6):604-13. doi: 10.7164/antibiotics.33.604.
The kinetics of gentamicin accumulation by a sensitive strain of Escherichia coli were investigated at gentamicin concentrations from 0.02 to 200 microgram/ml. Accumulation with time shows two energy-dependent phases and is saturable. Sodium chloride delays the onset of the second more rapid energy-dependent phase and decreases the magnitude of gentamicin accumulation for incubations up to 60 minutes at all gentamicin concentrations tested. Simultaneous determinations of accumulation, cell viability, and growth inhibition indicate that antimicrobial activity is correlated with the magnitude of gentamicin accumulation. These observations suggest that altered bacterial accumulation of gentamicin explains the effect of sodium chloride on the antimicrobial activity of gentamicin.
在庆大霉素浓度为0.02至200微克/毫升的条件下，研究了敏感大肠杆菌菌株对庆大霉素的积累动力学。随时间的积累呈现出两个能量依赖阶段，且具有饱和性。氯化钠会延迟第二个更快的能量依赖阶段的开始，并在所有测试的庆大霉素浓度下，使长达60分钟的孵育中庆大霉素的积累量降低。对积累、细胞活力和生长抑制的同步测定表明，抗菌活性与庆大霉素的积累量相关。这些观察结果表明，细菌对庆大霉素积累的改变解释了氯化钠对庆大霉素抗菌活性的影响。
