大肠杆菌甘油转运蛋白的底物特异性和转运特性
Substrate specificity and transport properties of the glycerol facilitator of Escherichia coli.
作者信息
Heller K B, Lin E C, Wilson T H
出版信息
J Bacteriol. 1980 Oct;144(1):274-8. doi: 10.1128/jb.144.1.274-278.1980.
Abstract
The specificity of the glycerol facilitator (glpF) of Escherichia coli was studied with an osmotic method. This transport system allowed the entry of polyols (glycerol and erythritol), pentitols, and hexitols. The analogous sugars were not transported. However, urea, glycine, and DL-glyceraldehyde could use this pathway to enter the cell. The glpF protein allowed the rapid efflux of preequilibrated xylitol. Glycerol surprisingly did not inhibit the uptake of xylitol, and xylitol only slightly reduced the uptake of glycerol. The observation and the insensitivity of the xylitol transport to low temperature suggest that the facilitator behaves as a membrane channel.
摘要
采用渗透法研究了大肠杆菌甘油转运蛋白（glpF）的特异性。该转运系统允许多元醇（甘油和赤藓糖醇）、戊糖醇和己糖醇进入细胞。类似的糖类则不能被转运。然而，尿素、甘氨酸和DL-甘油醛可以利用该途径进入细胞。glpF蛋白能使预平衡的木糖醇快速外流。令人惊讶的是，甘油并不抑制木糖醇的摄取，而木糖醇仅略微降低甘油的摄取。木糖醇转运对低温不敏感这一观察结果表明，该转运蛋白表现为一种膜通道。
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本文引用的文献
1
MEASUREMENT OF LOW ENERGY BETA-EMITTERS IN AQUEOUS SOLUTION BY LIQUID SCINTILLATION COUNTING OF EMULSIONS.通过乳液的液体闪烁计数法测量水溶液中的低能β发射体。
Anal Chem. 1965 Jun;37:854-7. doi: 10.1021/ac60226a017.
2
Utilization of L-alpha-glycerophosphate by Escherichia coli without hydrolysis.大肠杆菌对L-α-甘油磷酸的非水解利用。
Proc Natl Acad Sci U S A. 1962 Dec 15;48(12):2145-50. doi: 10.1073/pnas.48.12.2145.
3
[Reversible specific concentration of amino acids in Escherichia coli].[大肠杆菌中氨基酸的可逆特异性浓度]
Ann Inst Pasteur (Paris). 1956 Nov;91(5):693-720.
4
Periplasmic maltose-binding protein confers specificity on the outer membrane maltose pore of Escherichia coli.周质麦芽糖结合蛋白赋予大肠杆菌外膜麦芽糖孔特异性。
J Bacteriol. 1980 Feb;141(2):431-5. doi: 10.1128/jb.141.2.431-435.1980.
5
Specificity of diffusion channels produced by lambda phage receptor protein of Escherichia coli.大肠杆菌λ噬菌体受体蛋白产生的扩散通道的特异性
Proc Natl Acad Sci U S A. 1980 Jan;77(1):167-71. doi: 10.1073/pnas.77.1.167.
6
Control of permeation to glycerol in cells of Escherichia coli.大肠杆菌细胞中对甘油渗透的控制。
Biochem Biophys Res Commun. 1968 Jul 26;32(2):344-9. doi: 10.1016/0006-291x(68)90392-6.
7
Osmotically induced volume and turbidity changes of Escherichia coli due to salts, sucrose and glycerol, with particular reference to the rapid permeation of glycerol into the cell.由盐、蔗糖和甘油引起的大肠杆菌渗透压诱导的体积和浊度变化，特别提及甘油快速渗透进入细胞的情况。
J Gen Microbiol. 1974 May;82(1):125-42. doi: 10.1099/00221287-82-1-125.
8
Importance of facilitated diffusion for effective utilization of glycerol by Escherichia coli.协助扩散对大肠杆菌有效利用甘油的重要性。
J Bacteriol. 1972 Nov;112(2):784-90. doi: 10.1128/jb.112.2.784-790.1972.
9
Glycerol dissimilation and its regulation in bacteria.细菌中的甘油异化作用及其调控
Annu Rev Microbiol. 1976;30:535-78. doi: 10.1146/annurev.mi.30.100176.002535.
10
Outer membrane of gram-negative bacteria. XII. Molecular-sieving function of cell wall.革兰氏阴性菌的外膜。十二、细胞壁的分子筛功能。
J Bacteriol. 1976 Oct;128(1):325-36. doi: 10.1128/jb.128.1.325-336.1976.
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