在大肠杆菌中，甘油转运蛋白GlpF会积累锑酸盐。
Antimonite is accumulated by the glycerol facilitator GlpF in Escherichia coli.
作者信息
Sanders O I, Rensing C, Kuroda M, Mitra B, Rosen B P
机构信息
Department of Biochemistry and Molecular Biology, Wayne State University School of Medicine, Detroit, Michigan 48201, USA.
出版信息
J Bacteriol. 1997 May;179(10):3365-7. doi: 10.1128/jb.179.10.3365-3367.1997.
Abstract
In a search for genes responsible for the accumulation of antimonite in Escherichia coli, TnphoA was used to create a pool of random insertional mutants, from which one antimonite-resistant mutant was isolated. Sequence analysis showed that the TnphoA insertion was located in the glpF gene, coding for the glycerol facilitator GlpF. The mutant was shown to be defective in polyol transport by GlpF. These results suggest that in solution Sb(III) is recognized as a polyol by the glycerol facilitator.
摘要
为了寻找大肠杆菌中负责锑酸盐积累的基因，利用TnphoA创建了一个随机插入突变体库，从中分离出一个抗锑酸盐突变体。序列分析表明，TnphoA插入位于编码甘油转运蛋白GlpF的glpF基因中。该突变体在通过GlpF进行多元醇转运方面存在缺陷。这些结果表明，在溶液中Sb(III)被甘油转运蛋白识别为一种多元醇。
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