两种方法制备的大肠杆菌外膜多肽组成的比较。
Comparison of the polypeptide composition of Escherichia coli outer membranes prepared by two methods.
作者信息
Chopra I, Shales S W
出版信息
J Bacteriol. 1980 Oct;144(1):425-7. doi: 10.1128/jb.144.1.425-427.1980.
Abstract
Escherichia coli outer membranes were prepared by centrifugation to equilibrium in sucrose gradients and then treated with Sarkosyl in the presence of ethylenediaminetetraacetate. The polypeptide profiles of the two outer membrane preparations were compared by two-dimensional polyacrylamide gel electrophoresis. The patterns obtained were not identical, and Sarkosyl removed several minor proteins from the outer membrane.
摘要
通过在蔗糖梯度中离心至平衡来制备大肠杆菌外膜，然后在乙二胺四乙酸存在的情况下用十二烷基肌氨酸钠处理。通过二维聚丙烯酰胺凝胶电泳比较两种外膜制剂的多肽图谱。获得的图谱并不相同，并且十二烷基肌氨酸钠从外膜中去除了几种次要蛋白质。
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