Moriyon I, Berman D T
J Bacteriol. 1982 Nov;152(2):822-8. doi: 10.1128/jb.152.2.822-828.1982.
Cell envelopes prepared from smooth and rough strains of Brucella were characterized on the basis of lipopolysaccharide and protein content. The action of three kinds of detergents on Brucella cell envelopes and Escherichia coli control cell envelopes was examined on the basis of the proteins and lipopolysaccharides that were extracted. As compared with those of E. coli, Brucella cell envelopes were resistant to nonionic detergents. Zwittergents 312 and 316 were most effective in extracting E. coli cell envelopes, and Zwittergent 316 was most effective in extracting Brucella cell envelopes. Sarkosyl extracted proteins but extracted only trace amounts of lipopolysaccharides from cell envelopes of both bacteria. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of the Sarkosyl-resistant proteins revealed a composition similar to that of the proteins exposed on the surfaces of viable cells, as determined by the lactoperoxidase-125I radioiodination method. EDTA, with either Tris-HCl or Tris-HCl-Triton X-100, did not have detectable effects on Brucella cell envelopes. Ultracentrifugation of purified lipopolysaccharides in detergents and EDTA demonstrate that, in contrast to that of E. coli, Brucella lipopolysaccharide was not stabilized by divalent cations. Sarkosyl was ineffective in dispersing lipopolysaccharides, whereas the action of Zwittergents was related to the length of their alkyl chains.
从布鲁氏菌光滑型菌株和粗糙型菌株制备的细胞包膜，根据其脂多糖和蛋白质含量进行了表征。基于提取的蛋白质和脂多糖，研究了三种去污剂对布鲁氏菌细胞包膜和大肠杆菌对照细胞包膜的作用。与大肠杆菌相比，布鲁氏菌细胞包膜对非离子去污剂具有抗性。两性离子去污剂312和316在提取大肠杆菌细胞包膜方面最有效，而两性离子去污剂316在提取布鲁氏菌细胞包膜方面最有效。十二烷基肌氨酸钠能提取蛋白质，但从两种细菌的细胞包膜中仅提取痕量的脂多糖。对十二烷基肌氨酸钠抗性蛋白的十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分析显示，其组成与通过乳过氧化物酶-125I放射性碘化法测定的活细胞表面暴露的蛋白质组成相似。EDTA与Tris-HCl或Tris-HCl-Triton X-100一起使用时，对布鲁氏菌细胞包膜没有可检测到的影响。在去污剂和EDTA中对纯化的脂多糖进行超速离心表明，与大肠杆菌不同，布鲁氏菌脂多糖不受二价阳离子的稳定作用。十二烷基肌氨酸钠在分散脂多糖方面无效，而两性离子去污剂的作用与其烷基链的长度有关。
