酿酒酵母第五条染色体（CEN5）着丝粒的分离与特性分析

Isolation and characterization of the centromere from chromosome V (CEN5) of Saccharomyces cerevisiae.

作者信息

Maine G T, Surosky R T, Tye B K

出版信息

Mol Cell Biol. 1984 Jan;4(1):86-91. doi: 10.1128/mcb.4.1.86-91.1984.

DOI:10.1128/mcb.4.1.86-91.1984

PMID:6366514

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC368661/

Abstract

We have cloned a functional centromeric DNA sequence from Saccharomyces cerevisiae. Using the 2 mu chromosome-loss mapping technique and meiotic tetrad analysis, we have identified this DNA sequence as the centromere of chromosome V (CEN5). The CEN5 sequence has been localized on an 1,100-base-pair BamHI-BglII restriction fragment. Plasmids containing CEN5 and an autonomously replicating sequence are mitotically stable in S. cerevisiae and segregate in a Mendelian fashion during meiosis.

摘要

我们从酿酒酵母中克隆了一个功能性着丝粒DNA序列。利用2μm染色体丢失定位技术和减数分裂四分体分析，我们已将此DNA序列鉴定为第五条染色体（CEN5）的着丝粒。CEN5序列已定位在一个1100碱基对的BamHI - BglII限制性片段上。含有CEN5和自主复制序列的质粒在酿酒酵母中进行有丝分裂时是稳定的，并且在减数分裂过程中以孟德尔方式分离。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e00f/368661/c7a0d2616f43/molcellb00143-0102-a.jpg

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Cell. 1982 May;29(1):235-44. doi: 10.1016/0092-8674(82)90108-8.

Direct selection procedure for the isolation of functional centromeric DNA.

Proc Natl Acad Sci U S A. 1981 Jun;78(6):3760-4. doi: 10.1073/pnas.78.6.3760.

Genetic map of Saccharomyces cerevisiae.

Microbiol Rev. 1980 Dec;44(4):519-71. doi: 10.1128/mr.44.4.519-571.1980.

Autonomously replicating sequences in Saccharomyces cerevisiae.

Proc Natl Acad Sci U S A. 1980 Nov;77(11):6329-33. doi: 10.1073/pnas.77.11.6329.

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Nature. 1980 Oct 9;287(5782):504-9. doi: 10.1038/287504a0.

Chromosomes XIV and XVII of Saccharomyces cerevisiae constitute a single linkage group.

Mol Cell Biol. 1982 Nov;2(11):1399-409. doi: 10.1128/mcb.2.11.1399-1409.1982.

Centromeric DNA from Saccharomyces cerevisiae.

J Mol Biol. 1982 Jun 25;158(2):157-90. doi: 10.1016/0022-2836(82)90427-2.

Evidence for a new chromosome in Saccharomyces cerevisiae.

Mol Cell Biol. 1983 Mar;3(3):415-20. doi: 10.1128/mcb.3.3.415-420.1983.

Instability of dicentric plasmids in yeast.

Proc Natl Acad Sci U S A. 1983 Jan;80(1):228-32. doi: 10.1073/pnas.80.1.228.

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酿酒酵母第五条染色体（CEN5）着丝粒的分离与特性分析

Isolation and characterization of the centromere from chromosome V (CEN5) of Saccharomyces cerevisiae.

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