Kozel'tsev V L, Golubev V P, Volodina T V
Vopr Med Khim. 1980 Sep-Oct;26(5):680-5.
Composition of phospholipid preparations, isolated from rat liver mitochondria, was studied within various periods of incubation of the organelles obtained including 2 days interval. Distinct decrease in relative content of sphingomyelin, phosphatidyl choline and phosphatidyl ethanolamine was observed in the preparations during the incubation. At the same time, fractions of lysophospholipids and cardiolipin were markedly increased. Studies were carried out on the effect of the phospholipid fractions on hydrolysis of the protein component of mitochondrial membranes by lysosomal hydrolases and pronase. The phospholipids possessed protective functions in the system with the proteinases and mitochondrial membranes. The data obtained suggest that impairment of the phospholipid component appears to precede the proteolysis of proteins in mitochondrial membranes.
对从大鼠肝线粒体分离出的磷脂制剂在细胞器不同孵育时期（包括2天间隔）内的组成进行了研究。在孵育过程中，制剂中鞘磷脂、磷脂酰胆碱和磷脂酰乙醇胺的相对含量明显下降。同时，溶血磷脂和心磷脂的组分显著增加。研究了磷脂组分对溶酶体水解酶和链霉蛋白酶水解线粒体膜蛋白成分的影响。磷脂在蛋白酶与线粒体膜的系统中具有保护作用。所得数据表明，磷脂成分的损伤似乎先于线粒体膜中蛋白质的蛋白水解作用。
