Effect of phosphate on the arginine-induced insulin release by the isolated perfused rat pancreas.

The isolated perfused rat pancreas was used to investigate the effect of extracellular phosphate on the arginine-induced insulin release. In the absence of any metabolic substrate, the insulin response to arginine was monophasic. In the absence of phosphate in the medium, the insulin release as unaffected until the 15th minute of the stimulation period, but was significantly augmented from that time onward. In the presence of oleic acid in the perfusate, the insulin response to arginine was also monophasic but occurred earlier than in controls. In this conditin, phosphate omission resulted in an increase of the insulin response to arginine from the 3rd minute of the stimulatory period onward. In the presence of glucose 5.5 mM in the medium the insulin response to argnine was biphasic and was not affected by extracellular phosphate omission.