流式细胞术在肿瘤生物学中的应用：前景与陷阱。
Flow cytometric applications to tumour biology: prospects and pitfalls.
作者信息
Raju M R, Johnson T S, Tokita N, Gillette E L
出版信息
Br J Cancer Suppl. 1980 Apr;4:171-6.
Abstract
A brief review of cytometry instrumentation and its potential applications in tumour biology is presented using our recent data. DNA distribution measurements of cells from spontaneous dog tumours and cultured cells after exposure to X-rays, alpha particles or adriamycin are shown. The data show that DNA fluorescence measurments have application in the study of cell kinetics after either radiation or drug treatment. Extensive and careful experimentation is needed, however, to utilize the sophisticated developments in flow cytometry instrumentation.
摘要
利用我们最近的数据，对细胞计数仪器及其在肿瘤生物学中的潜在应用进行了简要综述。展示了来自自发犬肿瘤细胞以及暴露于X射线、α粒子或阿霉素后的培养细胞的DNA分布测量结果。数据表明，DNA荧光测量在辐射或药物治疗后的细胞动力学研究中具有应用价值。然而，要利用流式细胞术仪器的先进发展，还需要进行广泛而细致的实验。
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本文引用的文献
1
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Proc R Soc Med. 1966 Oct;59(10):979-82. doi: 10.1177/003591576605901020.
2
Cell-cycle analysis in 20 minutes.20分钟内完成细胞周期分析。
Science. 1974 Jun 21;184(4143):1297-8. doi: 10.1126/science.184.4143.1297.
3
A flow-system multiangle light-scattering instrument for cell characterization.一种用于细胞表征的流动系统多角度光散射仪。
Clin Chem. 1975 Aug;21(9):1297-304.
4
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Blood. 1976 Aug;48(2):245-58.
5
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Exp Cell Res. 1979 Sep;122(2):426-9. doi: 10.1016/0014-4827(79)90325-2.
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8
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