细胞阻抗特性的流动系统测量。

Flow-system measurement of cell impedance properties.

作者信息

Hoffman R A, Britt W B

出版信息

J Histochem Cytochem. 1979 Jan;27(1):234-40. doi: 10.1177/27.1.374580.

DOI:10.1177/27.1.374580

Abstract

A flow-system technique has been developed that detects the high-frequency resistance and capacitance changes in a sensing orifice due to the passage of cells through the orifice. The resistance and capacitance changes are related to cell properties such as size, plasma membrane capacitance, and electrical resistivity of the cell interior. The relationship between measured impedances and cellular properties is discussed, and the prototype instrument for making such measurements is described. The instrument can simultaneously detect the dc Coulter volume and two ac parameters related to the complex ac impedance change. Some initial tests of the instrument using plastic microspheres and Chinese hamster ovary cells are described.

摘要

已开发出一种流动系统技术，该技术可检测由于细胞通过传感孔而导致的传感孔中高频电阻和电容的变化。电阻和电容的变化与细胞特性相关，如大小、质膜电容和细胞内部的电阻率。讨论了测量阻抗与细胞特性之间的关系，并描述了进行此类测量的原型仪器。该仪器可同时检测直流库尔特体积和与复交流阻抗变化相关的两个交流参数。描述了使用塑料微球和中国仓鼠卵巢细胞对该仪器进行的一些初步测试。