Levine R F
Br J Haematol. 1980 Jul;45(3):487-97. doi: 10.1111/j.1365-2141.1980.tb07168.x.
Human megakaryocytes have been isolated from marrow obtained from ribs removed at thoracotomy. All but one of the patients had normal pre-operative platelet and leucocyte counts. Megakaryocytes averaged 0.37% of all cells in marrow cell suspensions from nine consecutive subjects. A 283-fold purification (to 10.3%) was achieved by a density gradient centrifugation followed by two successive velocity sedimentations at unit gravity. The net yield, 12 800 megakaryocytes per specimen, was sufficient for many kinds of morphological study. Bright-field, phase contrast, and electron microscopy were used to characterize the younger and smaller megakaryocytes. Ploidy analyses were carried out on 100--235 megakaryocytes per specimen; 8N was the predominant ploidy class in isolated megakaryocyte populations from three individuals. The mean megakaryocyte diameter was 24 micrometers in three other specimens and the range was 10--48 micrometers. This data had a normal distribution and overlapped minimally with the size range of all other marrow cells. The presence of a distinct size threshold (at 11.5 micrometers) implied that size alone may be a sufficient objective criterion for identification of human megakaryocytes.
人类巨核细胞已从开胸手术中切除的肋骨骨髓中分离出来。除一名患者外，所有患者术前血小板和白细胞计数均正常。在连续9名受试者的骨髓细胞悬液中，巨核细胞平均占所有细胞的0.37%。通过密度梯度离心，然后在单位重力下连续进行两次速度沉降，实现了283倍的纯化（至10.3%）。每个样本的净产量为12800个巨核细胞，足以进行多种形态学研究。利用明场、相差和电子显微镜对较年轻和较小的巨核细胞进行表征。对每个样本中的100 - 235个巨核细胞进行倍性分析；8N是来自三个个体的分离巨核细胞群体中的主要倍性类别。在其他三个样本中，巨核细胞的平均直径为24微米，范围为10 - 48微米。该数据呈正态分布，与所有其他骨髓细胞的大小范围最小重叠。存在一个明显的大小阈值（11.5微米）意味着仅大小可能是鉴定人类巨核细胞的一个充分的客观标准。
