Kohlhaw G B, Hsu Y P, Lemmon R D, Petes T D
J Bacteriol. 1980 Nov;144(2):852-5. doi: 10.1128/jb.144.2.852-855.1980.
The repression of beta-isopropylmalate dehydrogenase, the LEU2 gene product, by leucine and leucine plus threonine was unaffected by the transposition of LEU2 from its original locus on chromosome III to a new locus within the ribosomal deoxyribonucleic acid gene cluster on chromosome XII. Since the expression of the LEU2 gene is probably controlled at a pretranslational level, we conclude that the recombinant plasmid used for transformation carries regulatory information in addition to LEU2 structural information.
亮氨酸以及亮氨酸加苏氨酸对β-异丙基苹果酸脱氢酶(LEU2基因产物)的抑制作用,不受LEU2从其位于Ⅲ号染色体上的原始位点转座至Ⅻ号染色体核糖体脱氧核糖核酸基因簇内新位点的影响。由于LEU2基因的表达可能在翻译前水平受到控制,我们得出结论,用于转化的重组质粒除了携带LEU2结构信息外,还携带调控信息。