Fung J, Köhler H
J Immunol. 1980 Nov;125(5):1998-2003.
Neonatal BALB/c mice, given small amounts of an anti-idiotype serum directed against the TEPC-15 idiotype, are chronically unresponsive to immunization with PC antigens. These mice recover from suppression slowly over a period of 10 mo, and their response is not of TEPC-15 idiotype. However, the PC-specific precursors, as analyzed by the splenic fragment culture technique, in 8-mo-old suppressed mice are normal with respect to their frequency and idiotype dominance. Furthermore, the PC-precursors in neonatally suppressed BALB/c are sensitive to tolerance induction, as are precursors from neonatal liver and spleen cells. When chronic suppressed mice are immunized with a novel TI-1 antigen, PC-LPS, shown to stimulate immature PC-specific precursors, their response to PC is 80% of TEPC-15 idiotype, whereas their response to a TI-2 PC antigen and to a TD PC antigen is not TEPC-15 dominant. These results indicate that the TEPC-15 positive B cells in neonatally idiotype suppressed mice are in a state of immaturity that resembles the developmental characteristics of normal neonatal BALB/c mice.
给新生BALB/c小鼠注射少量针对TEPC-15独特型的抗独特型血清后，它们对PC抗原免疫呈慢性无反应状态。这些小鼠在10个月的时间里缓慢从抑制状态恢复，且它们的反应不是TEPC-15独特型的。然而，通过脾细胞片段培养技术分析，8月龄受抑制小鼠中的PC特异性前体细胞在频率和独特型优势方面是正常的。此外，新生期受抑制的BALB/c小鼠中的PC前体细胞对耐受诱导敏感，新生肝和脾细胞来源的前体细胞也是如此。当用一种新型TI-1抗原PC-LPS（已证明可刺激未成熟的PC特异性前体细胞）对慢性受抑制小鼠进行免疫时，它们对PC的反应80%是TEPC-15独特型的，而它们对TI-2 PC抗原和TD PC抗原的反应不是以TEPC-15为主导的。这些结果表明，新生期独特型受抑制小鼠中的TEPC-15阳性B细胞处于一种未成熟状态，类似于正常新生BALB/c小鼠的发育特征。
