Mahana W, Guilbert B, Avrameas S
Département d'Immunologie, Institut Pasteur, Paris, France.
Clin Exp Immunol. 1987 Dec;70(3):538-45.
Antibodies against idiotypic determinants carried by a monoclonal polyspecific natural autoantibody were raised in rabbits and in syngeneic BALB/c mice. These anti-idiotypic antibodies were administered to newborn and to pregnant BALB/c mice and to MRL-lpr/lpr mice. Serial measurements of the idiotypes, naturally occurring autoantibodies, and antibodies obtained after antigenic stimulation were performed in the sera of the injected mice and in the offspring of pregnant mice. No idiotypic suppression was noted in newborn injected mice. Transient suppression of idiotypes recognized by the syngeneic anti-idiotypic antibody was noted in the offspring of pregnant mice injected with the rabbit polyclonal anti-idiotypic antiserum. No changes in naturally occurring autoantibodies or in antibodies appearing after antigenic stimulation were noted in BALB/c mice. In contrast, a significant decrease of spontaneously occurring anti-DNA antibodies was found in MRL-lpr/lpr mice treated with rabbit polyclonal anti-idiotypic antiserum. Furthermore in these mice a slight decrease of anti-TNP antibodies was also observed. These results suggest that anti-idiotypic antibodies directed against natural autoantibodies may play a regulatory role in the immune system; this role is more easily appreciated in mice suffering from immune dysregulation.
针对单克隆多特异性天然自身抗体所携带的独特型决定簇的抗体，在兔和同基因BALB/c小鼠中产生。将这些抗独特型抗体给予新生的和怀孕的BALB/c小鼠以及MRL-lpr/lpr小鼠。对注射小鼠的血清以及怀孕小鼠的后代进行了独特型、天然存在的自身抗体以及抗原刺激后获得的抗体的系列测量。在新生注射小鼠中未观察到独特型抑制。在注射兔多克隆抗独特型抗血清的怀孕小鼠的后代中，观察到同基因抗独特型抗体识别的独特型的短暂抑制。在BALB/c小鼠中，未观察到天然存在的自身抗体或抗原刺激后出现的抗体有变化。相比之下，在用兔多克隆抗独特型抗血清治疗的MRL-lpr/lpr小鼠中，发现自发产生的抗DNA抗体显著减少。此外，在这些小鼠中还观察到抗TNP抗体略有减少。这些结果表明，针对天然自身抗体的抗独特型抗体可能在免疫系统中发挥调节作用；在患有免疫失调的小鼠中，这种作用更容易理解。
