Watkins W B, Reddy S
J Reprod Fertil. 1980 Mar;58(2):411-4. doi: 10.1530/jrf.0.0580411.
Antibodies raised against purified ovine placental lactogen were used to demonstrate the cellular localization of the hormone in tissues of the ewe placenta and chorionic membranes by immunofluorescence and immunoperoxidase techniques. Although binucleate cells have been shown to appear as early as Day 16 of pregnancy, ovine placental lactogen was not detected in the trophoblast until Day 22, and in substnatial numbers of cells until Day 80, of gestation. In the more mature placenta, lactogen-immunoreactive material was demonstrated in (a) binucleate cells of the fetal trophoblast, (b) uninucleate cells associated with the maternal epithelium syncytium and (c) binucleate cells of the chorionic membranes.
用针对纯化的绵羊胎盘催乳素产生的抗体，通过免疫荧光和免疫过氧化物酶技术来证明该激素在母羊胎盘和绒毛膜组织中的细胞定位。尽管早在妊娠第16天就已显示双核细胞出现，但直到妊娠第22天才在滋养层中检测到绵羊胎盘催乳素，直到妊娠第80天才在大量细胞中检测到。在更成熟的胎盘中，催乳素免疫反应性物质在以下细胞中得到证实：(a) 胎儿滋养层的双核细胞；(b) 与母体上皮合体细胞相关的单核细胞；(c) 绒毛膜的双核细胞。
