针对大肠杆菌RNA聚合酶亚基的抗体，作为DNA和其他配体亚基特异性结合的探针。
Antibodies against the subunits of E. coli RNA polymerase as probes for subunit-specific binding of DNA and other ligands.
作者信息
Stender W
出版信息
Nucleic Acids Res. 1980 Mar 25;8(6):1405-20. doi: 10.1093/nar/8.6.1405.
Abstract
Antibodies against the isolated subunits alpha, beta, and beta' of DNA-dependent RNA polymerase from E. coli have been prepared. They have been used to compare the extent of antibody-binding, as measured by complement fixation, to the isolated subunits and to the intact enzyme, in the absence and presence of ligands, such as inhibitors, nucleotides, nucleosides, oligo- and poly-nucleotides, and DNA of different composition. In many cases the results show a subunit-specific dependence of complement fixation upon the presence of a ligand and suggest a functional topography of the interaction between the subunits alpha, beta, and beta' of RNA polymerase and defined nucleotide sequences and small ligands.
摘要
已经制备了针对大肠杆菌DNA依赖性RNA聚合酶分离的α、β和β'亚基的抗体。在不存在和存在配体（如抑制剂、核苷酸、核苷、寡核苷酸和多核苷酸以及不同组成的DNA）的情况下，这些抗体已被用于比较通过补体结合测定的抗体与分离亚基和完整酶的结合程度。在许多情况下，结果表明补体结合对配体存在具有亚基特异性依赖性，并提示RNA聚合酶的α、β和β'亚基与特定核苷酸序列和小配体之间相互作用的功能拓扑结构。
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