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1
Antibodies against the subunits of E. coli RNA polymerase as probes for subunit-specific binding of DNA and other ligands.针对大肠杆菌RNA聚合酶亚基的抗体，作为DNA和其他配体亚基特异性结合的探针。
Nucleic Acids Res. 1980 Mar 25;8(6):1405-20. doi: 10.1093/nar/8.6.1405.
2
Monoclonal antibodies as probes of the topological arrangement of the alpha subunits of Escherichia coli RNA polymerase.单克隆抗体作为大肠杆菌RNA聚合酶α亚基拓扑排列的探针。
Biochemistry. 1989 Apr 18;28(8):3299-305. doi: 10.1021/bi00434a027.
3
[Contacts of Escherichia coli RNA polymerase subunits with nucleotides of lacUV5 promoter].
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4
Subunit topography of RNA polymerase (E. coli) in the complex with DNA.与DNA结合的RNA聚合酶（大肠杆菌）的亚基拓扑结构。
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5
Subunit assembly and metabolic stability of E. coli RNA polymerase.大肠杆菌RNA聚合酶的亚基组装与代谢稳定性
Proteins. 1987;2(1):42-53. doi: 10.1002/prot.340020106.
6
Characterization of effects of anti-beta and anti-beta' monoclonal antibodies on the activity of the RNA polymerase from Escherichia coli.
Biochemistry. 1985 Jun 18;24(13):3240-5. doi: 10.1021/bi00334a025.
7
Digestion with matrix-bound proteases as a possible probe for the topography of the DNA-dependent RNA polymerase from Escherichia coli.用与基质结合的蛋白酶消化作为探测大肠杆菌中依赖DNA的RNA聚合酶拓扑结构的一种可能方法。
Eur J Biochem. 1975 May;54(1):45-53. doi: 10.1111/j.1432-1033.1975.tb04112.x.
8
Conformational changes of Escherichia coli RNA polymerase on binding of templates.
FEBS Lett. 1979 Jul 1;103(1):57-60. doi: 10.1016/0014-5793(79)81249-1.
9
The beta ' subunit of Escherichia coli RNA polymerase is not required for interaction with initiating nucleotide but is necessary for interaction with rifampicin.大肠杆菌RNA聚合酶的β'亚基对于与起始核苷酸的相互作用不是必需的，但对于与利福平的相互作用是必需的。
J Biol Chem. 2001 Apr 20;276(16):13308-13. doi: 10.1074/jbc.M011041200. Epub 2001 Jan 22.
10
Sequence-specific interaction of sigma subunit of E. coli RNA polymerase with DNA.大肠杆菌RNA聚合酶的σ亚基与DNA的序列特异性相互作用。
Nucleic Acids Res. 1980 Aug 11;8(15):3459-66. doi: 10.1093/nar/8.15.3459.

引用本文的文献

1
Sequence-specific interaction of sigma subunit of E. coli RNA polymerase with DNA.大肠杆菌RNA聚合酶的σ亚基与DNA的序列特异性相互作用。
Nucleic Acids Res. 1980 Aug 11;8(15):3459-66. doi: 10.1093/nar/8.15.3459.
2
A temperature-sensitive mutant of Escherichia coli affected in the alpha subunit of RNA polymerase.一种在RNA聚合酶α亚基上受影响的大肠杆菌温度敏感突变体。
Nucleic Acids Res. 1990 Jun 25;18(12):3445-50. doi: 10.1093/nar/18.12.3445.

本文引用的文献

1
In vivo effect of thiolutin on cell growth and macromolecular synthesis in Escherichia coli.硫藤黄菌素对大肠杆菌细胞生长和大分子合成的体内效应。
Antimicrob Agents Chemother. 1974 Sep;6(3):304-10. doi: 10.1128/AAC.6.3.304.
2
THE ROLE OF ALPHA AND BETA CHAINS IN THE IMMUNOCHEMICAL DIFFERENCE BETWEEN OXY AND DEOXY HUMAN HAEMOGLOBIN.α链和β链在人氧合血红蛋白与脱氧血红蛋白免疫化学差异中的作用
J Mol Biol. 1965 Apr;11:775-84. doi: 10.1016/s0022-2836(65)80035-3.
3
THE IMMUNOCHEMICAL DIFFERENCE BETWEEN HORSE OXY- AND DEOXYHAEMOGLOBIN.马氧合血红蛋白与脱氧血红蛋白之间的免疫化学差异。
J Mol Biol. 1964 Sep;9:785-8. doi: 10.1016/s0022-2836(64)80184-4.
4
Reconstitution of bacterial DNA-dependent RNA-polymerase from isolated subunits as a tool for the elucidation of the role of the subunits in transcription.从分离的亚基重构细菌DNA依赖性RNA聚合酶作为阐明亚基在转录中作用的工具。
FEBS Lett. 1970 Dec;11(3):165-168. doi: 10.1016/0014-5793(70)80519-1.
5
Immunological aspects of the binding of substrate analogues and polypeptide inhibitors to alpha-chymotrypsin.底物类似物和多肽抑制剂与α-胰凝乳蛋白酶结合的免疫学方面
Immunochemistry. 1970 Mar;7(3):289-303. doi: 10.1016/0019-2791(70)90167-9.
6
Inhibition of RNA polymerase by streptolydigin.链霉溶菌素对RNA聚合酶的抑制作用。
Nat New Biol. 1971 Apr 14;230(15):197-200. doi: 10.1038/newbio230197a0.
7
A new preparation method for dark-field electron microscopy of biomacromolecules.一种用于生物大分子暗场电子显微镜观察的新制备方法。
J Ultrastruct Res. 1971 Apr;35(1):147-67. doi: 10.1016/s0022-5320(71)80148-x.
8
Immunological studies of aspartate transcarbamylase. II. Effect of ligands on the conformation of the enzyme.
Biochemistry. 1968 Dec;7(12):4322-9. doi: 10.1021/bi00852a026.
9
Serological relatedness of bacterial deoxyribonucleic acid polymerases.细菌脱氧核糖核酸聚合酶的血清学相关性
J Bacteriol. 1973 Jan;113(1):18-23. doi: 10.1128/jb.113.1.18-23.1973.
10
Subunits of RNA polymerase in function and structure; Maturation in vitro of core enzyme from Escherichia coli.RNA聚合酶的亚基在功能和结构方面；大肠杆菌核心酶的体外成熟
J Mol Biol. 1974 Aug 15;87(3):523-40. doi: 10.1016/0022-2836(74)90102-8.

针对大肠杆菌RNA聚合酶亚基的抗体，作为DNA和其他配体亚基特异性结合的探针。

Antibodies against the subunits of E. coli RNA polymerase as probes for subunit-specific binding of DNA and other ligands.

作者信息

Stender W

出版信息

Nucleic Acids Res. 1980 Mar 25;8(6):1405-20. doi: 10.1093/nar/8.6.1405.

DOI:10.1093/nar/8.6.1405
PMID:7001367
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC324000/
Abstract

Antibodies against the isolated subunits alpha, beta, and beta' of DNA-dependent RNA polymerase from E. coli have been prepared. They have been used to compare the extent of antibody-binding, as measured by complement fixation, to the isolated subunits and to the intact enzyme, in the absence and presence of ligands, such as inhibitors, nucleotides, nucleosides, oligo- and poly-nucleotides, and DNA of different composition. In many cases the results show a subunit-specific dependence of complement fixation upon the presence of a ligand and suggest a functional topography of the interaction between the subunits alpha, beta, and beta' of RNA polymerase and defined nucleotide sequences and small ligands.

摘要

已经制备了针对大肠杆菌DNA依赖性RNA聚合酶分离的α、β和β'亚基的抗体。在不存在和存在配体（如抑制剂、核苷酸、核苷、寡核苷酸和多核苷酸以及不同组成的DNA）的情况下，这些抗体已被用于比较通过补体结合测定的抗体与分离亚基和完整酶的结合程度。在许多情况下，结果表明补体结合对配体存在具有亚基特异性依赖性，并提示RNA聚合酶的α、β和β'亚基与特定核苷酸序列和小配体之间相互作用的功能拓扑结构。