Smit J W, Sondag-Tschroots I R, Aaij C, Feltkamp T E, Feltkamp-Vroom T M
Ann Rheum Dis. 1980 Aug;39(4):381-6. doi: 10.1136/ard.39.4.381.
The antigenic substrate of the antiperinuclear factor (APF) in human buccal mucosa cells was studied by light microscopy, cytochemistry, and the immunofluorescence technique (IFT). The cytoplasmic granules against which the APF is directed stained basophilic in light-microscopical staining techniques. The granules did not show a positive reaction product by techniques in which special chemical components are stained, and no activity of lysosomal enzymes could be identified. The use of autoantibodies and other antisera directed to distinct tissues, components, or macromolecules did not resolve the character of the antigenic granules. In addition it was shown that human vaginal epithelial cells and cryostat sections of human and rabbit buccal and oesophageal mucosa incubated with APF-positive sera showed the same fluorescent granules in the IFT as human buccal mucosa cells. Cryostat sections of rabbit buccal and oesophageal mucosa were tested as an alternative substrate in the APF test. Since the specificity as well as the sensitivity decreased when these sections were used as a substrate, they are not suitable for diagnostic purposes.
采用光学显微镜、细胞化学和免疫荧光技术（IFT）研究了人颊黏膜细胞中抗核周因子（APF）的抗原底物。在光学显微镜染色技术中，APF所针对的细胞质颗粒呈嗜碱性染色。通过对特殊化学成分进行染色的技术，这些颗粒未显示出阳性反应产物，也未鉴定出溶酶体酶的活性。使用针对不同组织、成分或大分子的自身抗体和其他抗血清，无法确定抗原颗粒的特征。此外，研究表明，用APF阳性血清孵育的人阴道上皮细胞以及人和兔颊黏膜和食管黏膜的冷冻切片，在IFT中显示出与人颊黏膜细胞相同的荧光颗粒。兔颊黏膜和食管黏膜的冷冻切片作为APF试验的替代底物进行了检测。由于将这些切片用作底物时特异性和敏感性均降低，因此它们不适合用于诊断目的。
