Youinou P, Serre G
Laboratory of Immunology, Brest University Medical School Hospital, France.
Int Arch Allergy Immunol. 1995 Aug;107(4):508-18. doi: 10.1159/000237093.
Antiperinuclear factor (APF) and antikeratin antibody (AKA) have long been known to be associated with rheumatoid arthritis. Human buccal mucosa epithelial cells have hitherto been required as the substrate in the APF test, while AKAs are detected on rat esophagus sections, using an indirect immunofluorescence technique. These two autoantibodies proved to be interrelated. Cytoplasmic inclusions in buccal cells have presumptively been termed keratohyalin granules and the APF target antigen colocalizes exactly with that of antiprofilaggrin antibody within the perinuclear organelles. The latter protein has convincingly been identified as the genuine specificity of the so-called AKA.
长期以来，抗核周因子（APF）和抗角蛋白抗体（AKA）一直被认为与类风湿性关节炎有关。迄今为止，APF检测需要用人颊黏膜上皮细胞作为底物，而AKA则是在大鼠食管切片上采用间接免疫荧光技术进行检测。事实证明，这两种自身抗体相互关联。颊细胞中的胞质内含物被推测为透明角质颗粒，并且APF靶抗原与抗丝聚蛋白原抗体的靶抗原在核周细胞器内完全共定位。后一种蛋白已被确凿地鉴定为所谓AKA的真正特异性抗原。
