在宿主介导试验中进行药物与亚硝酸联合诱变的检测。

Testing of drugs for combined mutagenesis with sodium nitrite in the host-mediated assay.

作者信息

Braun R, Schöneich J, Ziebarth D

出版信息

Arch Toxicol Suppl. 1980;4:49-53. doi: 10.1007/978-3-642-67729-8_10.

Abstract

Mutagenic activity of the drugs analgine and aminophenazone was tested in the intraperitoneal and intrasanguine host-mediated assay after oral application together with equimolar doses of sodium nitrite. Salmonella typhimurium strain G46 was used as genetic indicator system; mice served as host animals for the bacteria. Analgine was found to be weakly mutagenic in the dose 2 mM/kg together with nitrite, aminophenazone was a strong mutagen in combination with nitrite in the doses 2 mM/kg and 0.2 mM/kg using the intrasanguine test with 1 h incubation of bacteria in the liver. In the intraperitoneal variant with 3 h incubation time of bacteria only aminophenazone was slightly mutagenic at the highest dose tested, 2 mM/kg. The relative nitrosation rate for aminophenazone was calculated by means of regression lines for mutagenic activity of dimethylnitrosamine and was found to be in the range of 2% in both systems for a dose of 2 mM/kg precursors.

摘要

在口服给予等摩尔剂量亚硝酸钠后，采用腹腔注射和体内宿主介导试验检测了安乃近和氨基比林的致突变活性。以鼠伤寒沙门氏菌G46菌株作为遗传指示系统；小鼠作为细菌的宿主动物。发现在2 mM/kg剂量下，安乃近与亚硝酸盐一起具有弱致突变性；在肝内细菌孵育1小时的体内试验中，氨基比林在2 mM/kg和0.2 mM/kg剂量下与亚硝酸盐联合时是强致突变剂。在细菌孵育3小时的腹腔注射试验中，仅在最高测试剂量2 mM/kg时，氨基比林有轻微致突变性。通过二甲基亚硝胺致突变活性的回归线计算氨基比林的相对亚硝化率，发现在两个系统中，对于2 mM/kg前体剂量，其相对亚硝化率在2%的范围内。