Mirakian R, Bottazzo G F, Doniach D
Clin Exp Immunol. 1980 Jul;41(1):33-42.
The presence of autoantibodies detected by immunofluorescence to single endocrine cells, of human duodenum is described in three groups of patients and two control groups. Of 173 coeliac cases, four had GIP cell antibodies, one had secretin cell antibodies and twenty-one reacted with both cell types. Of twelve tropical sprue sera, four reacted with the same two cells. Among fifty elderly diabetics treated with hypoglycaemic drugs, seven sera gave a positive cytoplasmic IFL on duodenal substrate. Four were identified as GIP cells by use of the appropriate hormone antiserum and three reactions were against cells distinct from those stained by anti-GIP, -secretin, -somatostatin, -glucagon and -gastrin. Additional gut hormone antisera will have to be tested to identify these APUD cells. Thirty blood donors and seventy-three sera from autoimmune endocrine patients gave entirely negative results on unfixed cryostat sections of duodenal mucosa. Although impaired GIP and secretin responses have been reported in coeliac disease, and abnormal GIP values were found in Type II diabetes, there is as yet no data to correlate these metabolic dificiencies with the presence of endocrine cell antibodies in the serum. These studies are in progress.
本文描述了在三组患者和两个对照组中，通过免疫荧光检测到的人十二指肠单个内分泌细胞自身抗体的存在情况。在173例乳糜泻患者中，4例有胃抑肽（GIP）细胞抗体，1例有促胰液素细胞抗体，21例与两种细胞类型均发生反应。在12例热带口炎性腹泻血清中，4例与相同的两种细胞发生反应。在50例接受降糖药物治疗的老年糖尿病患者中，7份血清在十二指肠标本上呈现阳性细胞质免疫荧光。通过使用适当的激素抗血清，4例被鉴定为GIP细胞，3例反应针对的细胞与抗GIP、促胰液素、生长抑素、胰高血糖素和胃泌素染色的细胞不同。必须测试额外的肠道激素抗血清以鉴定这些APUD细胞。30名献血者和73份自身免疫性内分泌疾病患者的血清在十二指肠黏膜未固定的低温切片上结果完全为阴性。尽管在乳糜泻中已报道有GIP和促胰液素反应受损，且在II型糖尿病中发现GIP值异常，但尚无数据将这些代谢缺陷与血清中内分泌细胞抗体的存在相关联。这些研究正在进行中。
