Backendorf C, Overbeek G P, Van Boom J H, Van Der Marel G, Veeneman G, Van Duin J
Eur J Biochem. 1980 Sep;110(2):599-604. doi: 10.1111/j.1432-1033.1980.tb04904.x.
The deoxyoctanuclotide (5'-3')d(A-A-G-G-A-G-G-T), which is complementary to the 3' end of 16-S RNA, inhibits the formation of the complex between the 30-S subunit and MS2 RNA described in the preceding paper. If the complex is preformed, the octanucleotide cannot prevent entry of the complex into the ribosome cycle upon supplementation with the components for protein synthesis. The subunit . MS2-RNA complex is unable to bind the octanucleotide. It is concluded that in the subunit . phage-RNA initiation precursor the 16-S terminus is base-paired with a complementary MS2 RNA sequence. Edeine, aurintricarboxylic acid and antibodies against ribosomal protein S1 prevent the association of phage RNA with 30-S subunits. These compounds do not, however, inhibit the binding of (5'-3')d(A-A-G-G-A-G-G-T) to 3-S subunits. It is concluded that formation of the complex between MS2 RNA and 30-S subunits does not depend solely on the Shine and Dalgarno base-paring reaction.
与16 - S RNA的3'端互补的脱氧八核苷酸（5'-3'）d（A - A - G - G - A - G - G - T）可抑制前一篇论文中所述的30 - S亚基与MS2 RNA之间复合物的形成。如果复合物预先形成，那么在补充蛋白质合成所需成分后，八核苷酸无法阻止该复合物进入核糖体循环。亚基。MS2 - RNA复合物无法结合八核苷酸。得出的结论是，在亚基。噬菌体 - RNA起始前体中，16 - S末端与互补的MS2 RNA序列碱基配对。放线菌素、金精三羧酸和抗核糖体蛋白S1的抗体可阻止噬菌体RNA与30 - S亚基结合。然而，这些化合物并不抑制（5'-3'）d（A - A - G - G - A - G - G - T）与3 - S亚基的结合。得出的结论是，MS2 RNA与30 - S亚基之间复合物的形成并不完全依赖于Shine和Dalgarno碱基配对反应。
