Schonfeld S E, Herles S
Immunology. 1980 Oct;41(2):475-81.
Enamel matrix proteins taken from neonatal C57Bl/6 mice were shown to be able to elicit in vitro proliferative responses in C57Bl/6 splenic lymphocytes taken from animals which had not been exposed to exogenous enamel proteins. Animals which had been injected with enamel matrix proteins in Freund's complete adjuvant made IgG antibodies against enamel proteins which were detected by indirect immunofluorescence. Spleen cells from the immune animals gave an augmented in vitro proliferative response to enamel proteins, while spleen cells from C57Bl/6 nu/nu mice or anti-Thy 1 and complement-treated normal C57Bl/6 spleen cells were incapable of responding to enamel proteins in vitro. Thus, enamel matrix proteins appear to be T-cell dependent autoantigens. The natural history of enamel matrix proteins is reviewed, and it is suggested, based on the anatomic details of enamel synthesis, secretion, and maturation, that enamel matrix proteins are autoantigenic because they are anatomically sequestered.
从新生C57Bl/6小鼠获取的釉基质蛋白，被证明能够在未接触过外源性釉质蛋白的C57Bl/6脾淋巴细胞中引发体外增殖反应。在弗氏完全佐剂中注射了釉基质蛋白的动物，产生了针对釉质蛋白的IgG抗体，该抗体通过间接免疫荧光检测到。免疫动物的脾细胞对釉质蛋白的体外增殖反应增强，而C57Bl/6裸鼠或经抗Thy 1和补体处理的正常C57Bl/6脾细胞的脾细胞在体外无法对釉质蛋白产生反应。因此，釉基质蛋白似乎是T细胞依赖性自身抗原。本文综述了釉基质蛋白的自然史，并基于釉质合成、分泌和成熟的解剖学细节提出，釉基质蛋白具有自身抗原性是因为它们在解剖学上被隔离。
