Głosnicka R, Gruszkiewicz E
Infect Immun. 1980 Nov;30(2):506-12. doi: 10.1128/iai.30.2.506-512.1980.
Purification of the envelope antigen of Yersinia pestis EV with passive hemagglutination activity is described. The purification procedure consisted of pancreatin digestion, chromatography on human erythrocyte stroma set on Celite, and rechromatography on Sephadex G-200. Chemical, physical, and biological properties of this antigen were investigated. The results show the lipid-polysaccharide structure of the isolated antigen. The carbohydrate moiety of the galactolipid antigen consists of galactose and fucose. The lipid fraction contained phosphatidylethanolamine and phosphatidylserine. The preparation showed high specificity in the hemagglutination reaction and in Y. pestis phage receptor activity. In two-dimensional immunoelectrophoresis, the isolated pancreatic envelope digest antigen appeared as a single line. Two-dimensional immunoelectrophoresis was modified for tandem separation and was employed to electrophoretically identify the pancreatic envelope digest, trypsin envelope digest preparation, and F1 envelope antigen of Y. pestis. Related or identical antigens showed confluence of peaks with reactions of identity.
描述了具有被动血凝活性的鼠疫耶尔森氏菌EV包膜抗原的纯化方法。纯化步骤包括胰蛋白酶消化、在硅藻土上的人红细胞基质柱色谱以及在葡聚糖凝胶G-200上的再色谱。研究了该抗原的化学、物理和生物学性质。结果显示了分离抗原的脂多糖结构。半乳糖脂抗原的碳水化合物部分由半乳糖和岩藻糖组成。脂质部分含有磷脂酰乙醇胺和磷脂酰丝氨酸。该制剂在血凝反应和鼠疫耶尔森氏菌噬菌体受体活性方面表现出高特异性。在二维免疫电泳中，分离的胰包膜消化抗原呈现为单一泳道。对二维免疫电泳进行了改进以进行串联分离，并用于电泳鉴定鼠疫耶尔森氏菌的胰包膜消化物、胰蛋白酶包膜消化物制剂和F1包膜抗原。相关或相同的抗原在同一性反应中显示出峰的汇合。
