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鼠疫耶尔森氏菌脂多糖的化学和物理性质

Chemical and physical properties of lipopolysaccharide of Yersinia pestis.

作者信息

Hartley J L, Adams G A, Tornabene T G

出版信息

J Bacteriol. 1974 Jun;118(3):848-54. doi: 10.1128/jb.118.3.848-854.1974.

DOI:10.1128/jb.118.3.848-854.1974
PMID:4829929
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC246832/
Abstract

Lipopolysaccharide (LPS) prepared from Yersinia pestis 195/P contained d-glucose, d-glycero-d-mannoheptose, l-glycero-d-mannoheptose, glucosamine, 3-deoxyoctulosonic acid, lipid A, beta-hydroxymyristate, acetyl, phosphate, and protein. Traces of ethanolamine, mannose, and galactose were also detected. The lipid A moiety was composed of glucosamine substituted with phosphate, amide-linked beta-hydroxymyristate, and amide-bound acetate. The absence of significant amounts of additional fatty acids indicates a lipid A structure somewhat less complex than that of other gram-negative bacteria. The sugars identified are those generally found in the "core" region of LPS from the Enterobacteriaceae, with the exception of the d-glycero-d-mannoheptose. The molecular weight of the aggregated LPS was estimated to be 1.6 x 10(8).

摘要

从鼠疫耶尔森氏菌195/P制备的脂多糖(LPS)含有d-葡萄糖、d-甘油-d-甘露庚糖、l-甘油-d-甘露庚糖、葡糖胺、3-脱氧辛酮酸、脂质A、β-羟基肉豆蔻酸、乙酰基、磷酸盐和蛋白质。还检测到痕量的乙醇胺、甘露糖和半乳糖。脂质A部分由被磷酸盐取代的葡糖胺、酰胺连接的β-羟基肉豆蔻酸和酰胺结合的乙酸盐组成。大量其他脂肪酸的缺失表明脂质A结构比其他革兰氏阴性菌的结构稍简单。鉴定出的糖类是通常在肠杆菌科细菌LPS的“核心”区域中发现的那些糖类,但d-甘油-d-甘露庚糖除外。聚集的LPS的分子量估计为1.6×10⁸ 。