Arntzen L, Wadee A A, Isaäcson M
Department of Tropical Diseases, School of Pathology, University of the Witwatersrand, Johannesburg, South Africa.
Infect Immun. 1991 Jun;59(6):1966-71. doi: 10.1128/iai.59.6.1966-1971.1991.
This study assessed the in vitro cell-mediated immune responses of Mastomys natalensis, with a diploid chromosome number of 2n = 32, and Mastomys coucha, with a diploid chromosome number of 2n = 36, to Yersinia pestis. Splenic mononuclear (MN) cells of uninfected M. natalensis proliferated in response to crude fraction 1 of Y. pestis and two subfractions derived from fraction 1 in vitro. Proliferation was dose dependent and followed the time kinetics of other well-known mitogens. Further characterization of the two fractions revealed similar protein profiles in sodium dodecyl sulfide-polyacrylamide gel electrophoresis and indicated a heat-stable protein of 25 kDa responsible for the mitogenic activity. No such response was observed with MN cells from M. coucha. The unresponsiveness of M. coucha-derived MN cells appears to be related to an inability to respond to Y. pestis organisms. The results may help explain the relative resistance and susceptibility of M. natalensis and M. coucha to Y. pestis infection.
本研究评估了二倍体染色体数为2n = 32的南非多乳鼠和二倍体染色体数为2n = 36的库氏多乳鼠对鼠疫耶尔森菌的体外细胞介导免疫反应。未感染的南非多乳鼠的脾单核（MN）细胞在体外对鼠疫耶尔森菌的粗提物1及其衍生的两个亚组分有增殖反应。增殖呈剂量依赖性，并遵循其他知名有丝分裂原的时间动力学。对这两个组分的进一步表征显示，在十二烷基硫酸钠-聚丙烯酰胺凝胶电泳中蛋白质谱相似，并表明一种25 kDa的热稳定蛋白具有促有丝分裂活性。库氏多乳鼠的MN细胞未观察到这种反应。库氏多乳鼠来源的MN细胞无反应似乎与无法对鼠疫耶尔森菌产生反应有关。这些结果可能有助于解释南非多乳鼠和库氏多乳鼠对鼠疫耶尔森菌感染的相对抗性和易感性。
