Messiha F S
Int J Androl. 1980 Aug;3(4):375-82. doi: 10.1111/j.1365-2605.1980.tb00127.x.
The distribution of (newly found) NAD-dependent aldehyde dehydrogenase (ALDH) was determined in the rat testis and epididymis as a function of age. There were changes in specific activity of testicular ALDH as a function of age. Epididymal ALDH, which is found evenly distributed between the caput and cauda epididymis, increased initially from the 20th day after birth to th 50th day and remained little altered thereafter in both the caudet and the caput portion of the epididymis. Testicular and epididymal ALDH were found species-dependent with the hamster testis and the rat epididymis showing greater specific activities from the rest of the species studied, respectively. Determinations of testicular ALDH in various mouse strains indicate that it is strain-dependent. Intake of 25% (v/v) ethanol solution as the sole drinking fluid for 10 consecutive days inhibited testicular ALDH in the three mouse strains studied. Administration of disulfiram, 15 mg/kg/day for 60 consecutive days, exerted inhibitory action on testicular and epididymal ALDH in the rat. Intraperitoneal injection of pyrazol, 100 mg/kg once daily for 7 days, resulted in inhibition of epididymal but not of testicular ALDH. It is suggested that testicular and epididymal ALDH may play a role in the toxic action of ethanol-derived acetaldehyde on the gonad.
研究了（新发现的）NAD 依赖性醛脱氢酶（ALDH）在大鼠睾丸和附睾中的分布随年龄的变化情况。睾丸 ALDH 的比活性随年龄而变化。附睾 ALDH 在附睾头和附睾尾之间分布均匀，出生后第 20 天到第 50 天其活性最初增加，此后在附睾尾和附睾头部分均变化不大。睾丸和附睾 ALDH 具有物种依赖性，仓鼠睾丸和大鼠附睾的比活性分别高于所研究的其他物种。对各种小鼠品系的睾丸 ALDH 测定表明其具有品系依赖性。连续 10 天以 25%（v/v）乙醇溶液作为唯一饮用液体，抑制了所研究的三种小鼠品系的睾丸 ALDH。连续 60 天每天给予双硫仑 15mg/kg，对大鼠的睾丸和附睾 ALDH 产生抑制作用。每天腹腔注射吡唑 100mg/kg，连续 7 天，导致附睾 ALDH 受到抑制，但睾丸 ALDH 未受抑制。提示睾丸和附睾 ALDH 可能在乙醇衍生的乙醛对性腺的毒性作用中发挥作用。
