Loewus M W, Loewus F A, Brillinger G U, Otsuka H, Floss H G
J Biol Chem. 1980 Dec 25;255(24):11710-2.
Experiments with D-glucose-6-P stereospecifically tritiated at C-6 showed that the myo-inositol-1 P synthase reaction catalyzed by both the enzyme from beef testis and from pollen of Lilium longiflorum proceeds with stereospecific loss of the pro-6R and incorporation of the pro-6S hydrogen into the product. The ring closure thus occurs in a retention mode at C-6 of the substrate, a finding at variance with an earlier report, but in agreement with the stereochemistry recently determined for the reaction in Streptomyces flavopersicus and with the general stereochemical mode of operation of aldolases.
对在C-6位置立体特异性氚标记的D-葡萄糖-6-磷酸进行的实验表明，由牛睾丸和麝香百合花粉中的酶催化的肌醇-1-磷酸合酶反应，在底物的C-6位置以立体特异性方式失去前-6R氢，并将前-6S氢掺入产物中。因此，环化反应在底物的C-6位置以保留模式发生，这一发现与早期报告不同，但与最近确定的黄褐链霉菌中该反应的立体化学以及醛缩酶的一般立体化学操作模式一致。
