Deverill I, Reeves W G
J Immunol Methods. 1980;38(3-4):191-204. doi: 10.1016/0022-1759(80)90267-7.
Fluid-phase immunoprecipitation analysis has undergone two distinct paths of development. One path utilizes existing absorptiometric equipment while the other relies on apparatus constructed specifically for the measurement of scattered light. The principles and instrumentation employed in light scattering and absorption techniques are reviewed with special reference to their applications in immunology. The main features of available analysers are summarised in the table. Specialised equipment is becoming increasingly sophisticated and expensive and it is important to assess critically the benefits, if any, that such apparatus may offer compared with more conventional devices. The latter are often capable of performing the same function with greater versatility. A good quality bench spectrophotometer is a more appropriate multi-purpose laboratory instrument than a specialised nephelometer with its more limited application. The equipment of choice for automated immunochemical protein quantitation, however, is the centrifugal analyser. The capital outlay is compensated for by high throughput, development potential and reduced consumption of reagents (i.e. antisera).
液相免疫沉淀分析经历了两条不同的发展路径。一条路径利用现有的吸光测量设备，而另一条则依赖专门为测量散射光而构建的仪器。本文回顾了光散射和吸收技术所采用的原理及仪器设备，并特别提及它们在免疫学中的应用。表中总结了现有分析仪的主要特点。专业设备正变得越来越复杂且昂贵，因此，与更传统的设备相比，严格评估此类仪器可能带来的益处（如果有的话）非常重要。后者通常能够以更高的通用性执行相同的功能。一台优质的台式分光光度计比应用较为有限的专业浊度计更适合作为多功能实验室仪器。然而，用于自动免疫化学蛋白质定量的首选设备是离心分析仪。其高产量、发展潜力以及试剂（即抗血清）消耗的减少弥补了资本支出。
