Dutta S K, Nelson W H, Blackman C F, Brusick D J
J Environ Pathol Toxicol. 1980 Jun-Jul;3(5-6):195-206.
Several tester strains of Salmonella typhimurium, TA-98, TA-100, TA-1535, and TA-1538; Escherichia coli, W3110 (pol A+) and p3438 (pol A-, repair deficient); and Saccharomyces cerevisiae, D3, D4, and D5 were tested for lethal and mutagenic events when exposed to elevated temperatures or to x-band, pulsed microwave radiation at various power densities. When compared to E. coli pol A+ under growing conditions, E. coli pol A- exhibited decreased cell growth when exposed to microwave radiation at power levels at or above 20 mW/cm2 as well as to temperature levels above 42 degrees C. All yeast and other bacterial strains showed cellular lethality at similar microwave intensities and elevated temperatures. When exposed to elevated temperatures in saline, both quiescent yeast and Salmonella strains exhibited lethal events. However, the Salmonella strains tested showed comparatively less induction of genetic events in the quiescent state compared to induction when the cell were actively growing in broth. These results demonstrate that elevated temperatures generated by microwave exposure could produce genetic events in microbial assay systems. If such systems are to be of value in examining the nonthermal genetic potential of microwave radiation, careful control over exposure conditions will be required to eliminate heat-induced genetic events.
对几种测试菌株进行了测试，包括鼠伤寒沙门氏菌TA-98、TA-100、TA-1535和TA-1538；大肠杆菌W3110（pol A+）和p3438（pol A-，修复缺陷型）；以及酿酒酵母D3、D4和D5，观察它们在暴露于高温或不同功率密度的X波段脉冲微波辐射时的致死和诱变情况。与生长条件下的大肠杆菌pol A+相比，大肠杆菌pol A-在暴露于功率水平等于或高于20 mW/cm2的微波辐射以及高于42摄氏度的温度水平时，细胞生长出现下降。所有酵母和其他细菌菌株在相似的微波强度和高温下均表现出细胞致死性。当在盐溶液中暴露于高温时，静止的酵母和沙门氏菌菌株均出现致死情况。然而，与在肉汤中活跃生长的细胞相比，测试的沙门氏菌菌株在静止状态下遗传事件的诱导相对较少。这些结果表明，微波暴露产生的高温可在微生物检测系统中引发遗传事件。如果此类系统要在检测微波辐射的非热遗传潜力方面具有价值，将需要仔细控制暴露条件以消除热诱导的遗传事件。
