Gher M E, Williams J E, Vernino A R, Strong D M, Pelleu G B
J Periodontol. 1980 Oct;51(10):571-7. doi: 10.1902/jop.1980.51.10.571.
A study was undertaken to evaluate the antigenic potential of allogeneic freeze-dried skin (FDS) when used as a grafting material and to examine the clinical results when FDS is used for the correction of mucogingival problems in humans. FDS was obtained from an HLA-tissue-type donor according to Navy Tissue Bank protocol. Allogeneic FDS grafts were performed to correct mucogingival problems. In addition, periosteal fenestration was used in conjunction with approximately one-half of the grafts. Documentation with a standardized periodontal probe consisted of clinical measurements taken preoperatively and 8 weeks postoperatively. Blood samples were drawn prior to and at 1, 2, 3, 5, and 8 weeks postsurgically. Testing of blood samples for anti-HLA antibody was performed by use of a modified microcytotoxicity assay. Thirty-one patients received 36 FDS grafts involving 148 tooth sites. The FDS allografts resulted in a significant (P < 0.001) increase in the width of attached-type tissue (mean gain = 3.80 mm). Clinically, there was no sign of graft rejection, and serologically, all serum samples tested were nagative for the presence of anti-HLA antibody. FDS grafts used for the treatment of mucogingival problems in humans are nonimmunogenic as evaluated for anti-HLA antibody. The surgical procedure performed using FDS as a graft material resulted in a significant increase in the width of attached-type tissue.
本研究旨在评估同种异体冻干皮肤（FDS）作为移植材料时的抗原性潜力，并检验FDS用于纠正人类黏膜牙龈问题时的临床效果。根据海军组织库方案，从一名HLA组织类型供体获取FDS。进行同种异体FDS移植以纠正黏膜牙龈问题。此外，约一半的移植物联合使用了骨膜开窗术。使用标准化牙周探针记录术前及术后8周的临床测量数据。在手术前以及术后1、2、3、5和8周采集血样。通过改良的微量细胞毒性试验检测血样中的抗HLA抗体。31例患者接受了36次FDS移植，涉及148个牙位。FDS同种异体移植物使附着型组织宽度显著增加（P < 0.001）（平均增加3.80 mm）。临床上，未出现移植物排斥迹象，血清学检测显示，所有检测的血清样本抗HLA抗体均为阴性。就抗HLA抗体评估而言，用于治疗人类黏膜牙龈问题的FDS移植物无免疫原性。使用FDS作为移植材料进行的手术操作使附着型组织宽度显著增加。
