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使用同位素稀释-质谱法对临床化学中使用的不同常规方法进行准确性控制。

Use of isotope dilution--mass spectrometry for accuracy control of different routine methods used in clinical chemistry.

作者信息

Björkhem I, Blomstrand R, Eriksson S, Falk O, Kallner A, Svensson L, Ohman G

出版信息

Scand J Clin Lab Invest. 1980 Oct;40(6):529-34. doi: 10.3109/00365518009091960.

DOI:10.3109/00365518009091960
PMID:7003693
Abstract

Serum from patients was pooled, filtered, dispensed and frozen. The serum pool obtained was used for accuracy control in twenty-one participating laboratories in the Stockholm area. Mean values (state of art values) were obtained for creatinine, cholesterol, glucose, urea and uric acid. These values were compared with values obtained with highly accurate reference methods, based on isotope dilution--mass fragmentography. The most marked difference was found in the case of determination of creatinine. It is suggested that immediate access to such a well-defined human serum might decrease the need for conventional interlaboratory control programmes.

摘要

将患者的血清汇集、过滤、分装并冷冻。所获得的混合血清用于斯德哥尔摩地区21个参与实验室的准确性控制。得出了肌酐、胆固醇、葡萄糖、尿素和尿酸的均值(现有技术值)。将这些值与基于同位素稀释-质谱碎片分析法的高精度参考方法所获得的值进行比较。在肌酐测定方面发现了最显著的差异。建议能即时获取如此定义明确的人血清可能会减少对传统实验室间控制程序的需求。

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