Björkhem I, Bergman A, Falk O, Kallner A, Lantto O, Svensson L, Akerlöf E, Blomstrand R
Clin Chem. 1981 May;27(5):733-5.
Serum from patients was pooled, filtered, dispensed, and frozen. This pooled specimen was used for accuracy control in 64 participating laboratories in Sweden. Mean values ("state-of-the-art" values) were obtained for creatinine, cholesterol, glucose, urea, uric acid, and cortisol. These values were compared with values obtained with highly accurate reference methods based on isotope dilution-mass spectrometry. Differences were marked in the case of determination of creatinine and cortisol. Concerning the other components, the differences between the state-of-the-art value and the values obtained with the reference methods were negligible. Moreover, the glucose oxidase and the oxime methods for determination of glucose and urea were found to give significantly lower values than the hexokinase and urease methods, respectively. We conclude that methods with a higher degree of accuracy are required for routine determination of creatinine and cortisol.
将患者的血清汇集、过滤、分装并冷冻。这个汇集样本用于瑞典64个参与实验室的准确性控制。获得了肌酐、胆固醇、葡萄糖、尿素、尿酸和皮质醇的平均值（“最新技术”值）。将这些值与基于同位素稀释-质谱法的高精度参考方法所获得的值进行比较。在肌酐和皮质醇的测定中差异明显。关于其他成分，最新技术值与参考方法所获得的值之间的差异可以忽略不计。此外，发现用于测定葡萄糖和尿素的葡萄糖氧化酶法和肟法分别比己糖激酶法和脲酶法给出的值显著更低。我们得出结论，肌酐和皮质醇的常规测定需要更高准确度的方法。
