诺维科夫肝癌染色质的免疫特异性：三种抗原蛋白的分离

Immunological specificity of Novikoff hepatoma chromatin: isolation of three antigenic proteins.

Stryjecka-Zimmer M, Schmidt W N, Briggs R C, Hnilica L S

Int J Biochem. 1982;14(7):591-600. doi: 10.1016/0020-711x(82)90042-8.

Abstract

Immunization of rabbits with dehistonized Novikoff hepatoma or normal rat liver chromatin elicited specific antibodies. 2. Chromosomal nonhistone proteins from Novikoff hepatoma were fractionated and three proteins (approx. mol. wt 39,000, 49,0000 and 56,000) were identified with the hepatoma antigenic complexes. 3. All three proteins showed significant reactivity of PAS staining suggesting that they were glycosylated. 4. Proteins migrating similar to these three Novikoff hepatoma fractions were detected by polyacrylamide gel electrophoresis in the chromosomal nonhistone protein fraction obtained from normal rat liver. 5. However, the three proteins from normal liver were PAS negative, and did not react immunologically with antiserum to dehistonized Novikoff hepatoma chromatin.

摘要

用去组蛋白的诺维科夫肝癌或正常大鼠肝脏染色质免疫兔子可产生特异性抗体。2. 对诺维科夫肝癌的染色体非组蛋白进行分级分离，鉴定出三种蛋白质（分子量约为39,000、49,000和56,000）与肝癌抗原复合物相关。3. 这三种蛋白质均显示出明显的PAS染色反应性，表明它们被糖基化。4. 通过聚丙烯酰胺凝胶电泳在从正常大鼠肝脏获得的染色体非组蛋白部分中检测到迁移情况与这三种诺维科夫肝癌组分相似的蛋白质。5. 然而，正常肝脏中的这三种蛋白质PAS染色呈阴性，并且与去组蛋白的诺维科夫肝癌染色质抗血清没有免疫反应。