诺维科夫肝癌抗原蛋白p39、p49和p56的组织特异性及细胞分布

Tissue specificity and cellular distribution of Novikoff hepatoma antigenic proteins p39, p49, and p56.

作者信息

Schmidt W N, Stryjecka-Zimmer M, Glass W F, Briggs R C, Hnilica L S

出版信息

J Biol Chem. 1981 Aug 10;256(15):8117-23.

Abstract

Three Novikoff hepatoma protein antigens (approximately Mr = 39,000, 49,000, and 56,000) were partially purified from Novikoff hepatoma chromatin. Rabbit antiserum to these three proteins was used to examine various rat tissues for the presence of these antigens. Immunological specificity of the antiserum was assessed using quantitative microcomplement fixation assay or by visualization of the immunoreactive complexes with peroxidase-antiperoxidase procedure after transferring the electrophoretically separated proteins to nitrocellulose sheets. The immunoreactivity was localized with the three proteins p39, p49, and p56 in Novikoff hepatoma. The p56 protein was found to be present in normal rat liver, 24-h regenerating rat liver, fetal rat liver, or kidney, albeit in much smaller amounts as found in Novikoff hepatoma. the p49 and p39 antigens were specific for Novikoff hepatoma. Immunoabsorption experiments confirmed the specificity of this antiserum. Assessment of various subcellular fractions of Novikoff hepatoma revealed that the p39, p49, and p56 protein antigens are present in the cytoplasmic fractions as well as in isolated chromatin.

摘要

从诺维科夫肝癌染色质中部分纯化出三种诺维科夫肝癌蛋白抗原（分子量约为39,000、49,000和56,000）。用针对这三种蛋白质的兔抗血清检测各种大鼠组织中是否存在这些抗原。使用定量微量补体结合试验或在将电泳分离的蛋白质转移到硝酸纤维素膜上后，通过过氧化物酶-抗过氧化物酶法观察免疫反应复合物来评估抗血清的免疫特异性。免疫反应性定位于诺维科夫肝癌中的三种蛋白质p39、p49和p56。发现p56蛋白存在于正常大鼠肝脏、24小时再生大鼠肝脏、胎鼠肝脏或肾脏中，尽管其含量比在诺维科夫肝癌中发现的要少得多。p49和p39抗原对诺维科夫肝癌具有特异性。免疫吸收实验证实了该抗血清的特异性。对诺维科夫肝癌的各种亚细胞组分的评估表明，p39、p49和p56蛋白抗原存在于细胞质组分以及分离的染色质中。