Hamberg H, Brunk U T, Ericsson J L, Jung B
Acta Pathol Microbiol Scand A. 1980 Jul;88(4):231-9. doi: 10.1111/j.1699-0463.1980.tb02491.x.
Cultured human glial cells were blocked in interphase (G1) by 24 h of serum starvation and subsequently subjected to 200 Gy, 8 MV X-radiation. Immediately following irradiation the cultures were transferred to serum-containig medium. Time-lapse cinemicrography performed during the next 48 h showed a profoundly disturbed motility with decreased ability for polarization and locomotion of the irradiated cells. Specimens fixed 24 and 48 h after irradiation and investigated by immunofluorescence with tubulin-antibodies and DNase/DNase-antibodies, and by whole cell transmission electron microscopy showed derangements in the distribution of microfilament bundles related to the cytoplasmic ramification of the irradiated cells, but otherwise no detectable alterations in structure or distribution of either microtubules or microfilaments. It is suggested that the alteration in the arrangement of filament bundles is of importance to the impaired locomotion of the irradiated cells.
培养的人神经胶质细胞通过24小时的血清饥饿被阻断在间期（G1期），随后接受200戈瑞的8兆伏X射线辐射。辐射后立即将培养物转移到含血清的培养基中。在接下来的48小时内进行的延时电影显微镜观察显示，受辐射细胞的运动受到严重干扰，极化和移动能力下降。在辐射后24小时和48小时固定的标本，通过用微管蛋白抗体和脱氧核糖核酸酶/脱氧核糖核酸酶抗体进行免疫荧光检测，以及通过全细胞透射电子显微镜观察，结果显示与受辐射细胞的细胞质分支相关的微丝束分布紊乱，但在微管或微丝的结构或分布方面未发现其他可检测到的改变。有人认为，丝束排列的改变对受辐射细胞运动受损至关重要。
