Pontius K I, Hawk W A
Am J Clin Pathol. 1980 Nov;74(5):620-9. doi: 10.1093/ajcp/74.5.620.
All malignant neoplastic cells of papillary carcinoma of the thyroid gland and the majority of follicular carcinoma cells do not demonstrate the presence of normal cytoplasmic microsomal antigen. Follicular adenomas contain cells that demonstrate microsomal antigen, as well as cells that do not. This contrasts with normal and goitrous thyroid glands, which show positive fluorescence of all follicle cells. A concomitant immunofluorescence and ultrastructural study was performed using five normal thyroid specimens, six nodular goiters, five follicular adenomas, four follicular carcinomas, and four papillary carcinomas. The tissues were stained by a sandwich technic with initial incubation with human serum containing high-titer antimicrosomal antibody followed by incubation with fluorescein-conjugated goat antihuman polyspecific serum. The fluorescence findings suggest loss or functional alteration of the normal microsomal antigen in malignant neoplastic cells and some benign neoplastic cells. Ultrastructural study of tissue blocks from the same specimens revealed no conclusive difference in the cytoplasmic organelles of benign and malignant cells. The conclusion is that the immunologic identity of neoplastic follicle cells, as compared with that of normal cells, is altered prior to observable ultrastructural alteration of cytoplasmic constituents.
甲状腺乳头状癌的所有恶性肿瘤细胞以及大多数滤泡癌细胞均未显示正常细胞质微粒体抗原的存在。滤泡性腺瘤包含显示微粒体抗原的细胞以及不显示微粒体抗原的细胞。这与正常甲状腺和甲状腺肿不同，正常甲状腺和甲状腺肿的所有滤泡细胞均显示阳性荧光。使用5个正常甲状腺标本、6个结节性甲状腺肿、5个滤泡性腺瘤、4个滤泡癌和4个乳头状癌进行了免疫荧光和超微结构同步研究。组织采用夹心技术染色，首先用含高滴度抗微粒体抗体的人血清孵育，然后用荧光素结合的山羊抗人多特异性血清孵育。荧光结果表明，恶性肿瘤细胞和一些良性肿瘤细胞中正常微粒体抗原缺失或功能改变。对相同标本的组织块进行超微结构研究，结果显示良性和恶性细胞的细胞质细胞器无决定性差异。结论是，与正常细胞相比，肿瘤滤泡细胞的免疫学特性在细胞质成分出现可观察到的超微结构改变之前就已发生改变。
