Doniach I, Perrin J
Clin Exp Immunol. 1973 May;14(1):77-90.
Fresh surgical material from forty-eight patients undergoing partial thyroidectomy for thyroid tumour was examined by immunofluorescence staining with antisera for the presence of thyroid-microsomal, mitochondrial and thyroglobulin antigens. Light microscopy on cryostat and paraffin-wax embedded sections was carried out in all cases. Electron microscopy was done on samples from thirteen tumours. Microsomal antigen was present in all normal thyroid tissue, in all eight colloid nodules, in twenty-three out of twenty-four follicular adenomas, in four out of six microangeioinvasive follicular carcinomas, in none of the six papillary, one anaplastic and three medullary carcinomas. Mitochondrial antigen was present in all tumours. Electron microscopy showed marked reduction or absence of microvesicles and other organelles in the papillary and anaplastic carcinomas, thus confirming the suggestion put forward by Goudie & McCallum (1963) that a negative microsomal reaction is associated with dedifferentiation. A negative reaction was also associated with tumours showing advanced oxyphil (Hürthle) cell metaplasia, i.e. total replacement of cytoplasmic organelles by proliferated mitochondria.
对48例因甲状腺肿瘤接受甲状腺部分切除术患者的新鲜手术材料，用抗血清进行免疫荧光染色，以检测甲状腺微粒体、线粒体和甲状腺球蛋白抗原的存在。所有病例均对低温恒温器切片和石蜡包埋切片进行了光学显微镜检查。对13个肿瘤的样本进行了电子显微镜检查。微粒体抗原存在于所有正常甲状腺组织、所有8个胶样结节、24个滤泡性腺瘤中的23个、6个微小血管浸润性滤泡癌中的4个，而在6个乳头状癌、1个未分化癌和3个髓样癌中均未发现。所有肿瘤中均存在线粒体抗原。电子显微镜显示乳头状癌和未分化癌中的微泡和其他细胞器明显减少或缺失，从而证实了古迪和麦卡勒姆（1963年）提出的微粒体阴性反应与去分化相关的观点。阴性反应也与显示晚期嗜酸性（许特莱）细胞化生的肿瘤有关，即增殖的线粒体完全取代了细胞质细胞器。
