Gauldie J, Lamontagne L, Horsewood P, Jenkins E
Am J Pathol. 1980 Dec;101(3):723-36.
Using horseradish peroxidase and fluorescence immunohistochemistry, alpha 1-antitrypsin (alpha 1AT) was demonstrated in normal mouse hepatocytes and pancreatic islet cells. All hepatocytes were positive; 1--3% stained intensely for alpha 1AT. These were located mainly in the periportal area as well as randomly distributed, both singly and in clusters, throughout the liver lobule. Nonparenchymal liver cells were negative for alpha 1AT. The type of hepatocyte cytoplasmic staining appears to after during ontogeny, changing from a localized granular to a diffuse pattern. The use of immunohistochemistry to demonstrate alpha 1AT in normal mouse liver allows us to examine the acute phase response at a cellular level.
利用辣根过氧化物酶和荧光免疫组织化学方法，在正常小鼠肝细胞和胰岛细胞中证实了α1抗胰蛋白酶（α1AT）的存在。所有肝细胞均呈阳性；1%至3%的细胞对α1AT染色强烈。这些细胞主要位于汇管区周围，也呈单个或成簇状随机分布于整个肝小叶。肝非实质细胞对α1AT呈阴性。肝细胞胞质染色类型在个体发育过程中似乎会发生变化，从局部颗粒状变为弥漫性模式。利用免疫组织化学方法在正常小鼠肝脏中证实α1AT，使我们能够在细胞水平上研究急性期反应。
