Baumann H, Latimer J J, Glibetic M D
Arch Biochem Biophys. 1986 Apr;246(1):488-93. doi: 10.1016/0003-9861(86)90495-9.
Mouse plasma contains two major protease inhibitors, alpha 1-protease inhibitor (alpha 1-PI) and contrapsin, which have high affinity for bovine trypsin. Systemic injury, such as turpentine-induced inflammation, did not change the plasma concentration of alpha 1-PI, but increased that of contrapsin by 50%. The concentration of hepatic alpha 1-PI mRNA was determined by Northern blot hybridization and was not significantly affected by the acute phase reaction. J.M. Frazer, S.A. Nathoo, J. Katz, T.L. Genetta, and T.H. Finley [1985) Arch. Biochem. Biophys. 239, 112-119) have reported a threefold increase of mRNA for the elastase specific alpha 1-PI but this increase was not demonstrated by the present study. The mRNAs for known mouse acute phase plasma proteins were, however, stimulated severalfold by the same treatment. These results indicate that in the mouse, as opposed to human, alpha 1-PI is not an acute phase reactant.
小鼠血浆含有两种主要的蛋白酶抑制剂，即α1-蛋白酶抑制剂（α1-PI）和抗胰蛋白酶，它们对牛胰蛋白酶具有高亲和力。全身性损伤，如松节油诱导的炎症，并未改变α1-PI的血浆浓度，但使抗胰蛋白酶的血浆浓度增加了50%。通过Northern印迹杂交测定肝脏α1-PI mRNA的浓度，其不受急性期反应的显著影响。J.M.弗雷泽、S.A.纳图、J.卡茨、T.L.杰内塔和T.H.芬利[1985年，《生物化学与生物物理学报》239卷，第112 - 119页]报道，弹性蛋白酶特异性α1-PI的mRNA增加了三倍，但本研究未证实这种增加。然而，相同处理可使已知小鼠急性期血浆蛋白的mRNA增加数倍。这些结果表明，与人类不同，在小鼠中α1-PI不是急性期反应物。
