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苯丙氨酸转运核糖核酸与大肠杆菌核糖体上信使核糖核酸之间的光诱导交联。

Photo-induced crosslinking between phenylalanine transfer RNA and messenger RNA on the Escherichia coli ribosome.

作者信息

Matzke A J, Barta A, Kuechler E

出版信息

Eur J Biochem. 1980 Nov;112(1):169-78. doi: 10.1111/j.1432-1033.1980.tb04998.x.

DOI:10.1111/j.1432-1033.1980.tb04998.x
PMID:7004867
Abstract

Crosslinking between phenylalanyl transfer RNA from brewer's yeast and poly(U) can be obtained when ribosomal complexes are irradiated at > 300 nm; the ribosomal complexes were formed by enzymatic binding of the tRNA. The crosslinked product was isolated on oligo(dA)-cellulose columns. The experiments suggest that the photo-crosslinking occurs via the wybutine in the anticodon loop of the tRNA. This system can be used to study interactions between tRNA and messenger RNA on the ribosome.

摘要

当核糖体复合物在波长大于300纳米的光线下照射时，可实现来自酿酒酵母的苯丙氨酰转移核糖核酸（tRNA）与聚尿苷酸（poly(U)）之间的交联；核糖体复合物是通过tRNA的酶促结合形成的。交联产物在寡聚脱氧腺苷酸（oligo(dA)）-纤维素柱上分离。实验表明，光交联是通过tRNA反密码子环中的怀丁碱发生的。该系统可用于研究核糖体上tRNA与信使核糖核酸（mRNA）之间的相互作用。

相似文献

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Photo-induced crosslinking between phenylalanine transfer RNA and messenger RNA on the Escherichia coli ribosome.苯丙氨酸转运核糖核酸与大肠杆菌核糖体上信使核糖核酸之间的光诱导交联。
Eur J Biochem. 1980 Nov;112(1):169-78. doi: 10.1111/j.1432-1033.1980.tb04998.x.
2
Mechanism of translocation: relative arrangement of tRNA and mRNA on the ribosome.易位机制：tRNA与mRNA在核糖体上的相对排列。
Proc Natl Acad Sci U S A. 1980 Sep;77(9):5110-4. doi: 10.1073/pnas.77.9.5110.
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Specificity of the photo-crosslinking reaction between poly(U) and protein S1 on the Escherichia coli ribosome.大肠杆菌核糖体上聚（U）与蛋白质S1之间光交联反应的特异性。
FEBS Lett. 1978 Apr 1;88(1):131-4. doi: 10.1016/0014-5793(78)80624-3.
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Crosslinking transfer RNA and messenger RNA at the ribosomal decoding region: identification of the site of reaction on the messenger RNA.核糖体解码区域交联转运RNA和信使RNA：信使RNA上反应位点的鉴定。
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Effect of ribosome binding and translocation on the anticodon of tRNAPhe as studied by wybutine fluorescence.通过怀丁荧光研究核糖体结合与易位对苯丙氨酰-tRNA反密码子的影响。
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Poly(4-thiouridylic acid) as messenger RNA and its application for photoaffinity labelling of the ribosomal mRNA binding site.聚（4-硫代尿苷酸）作为信使核糖核酸及其在核糖体信使核糖核酸结合位点光亲和标记中的应用。
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Fluorimetric study of the complex between yeast phenylalanyl-tRNA synthetase and tRNA-Phe. 1. Changes in the conformation of the enzyme and tRNA; modification of the Wybutine neighbourhood.酵母苯丙氨酰 - tRNA合成酶与tRNA - Phe复合物的荧光研究。1. 酶和tRNA构象的变化；怀丁碱邻域的修饰。
Eur J Biochem. 1980 Jan;103(1):145-53. doi: 10.1111/j.1432-1033.1980.tb04298.x.

引用本文的文献

1
Crosslinking transfer RNA and messenger RNA at the ribosomal decoding region: identification of the site of reaction on the messenger RNA.核糖体解码区域交联转运RNA和信使RNA：信使RNA上反应位点的鉴定。
Nucleic Acids Res. 1984 Nov 12;12(21):8181-91. doi: 10.1093/nar/12.21.8181.
2
Erythromycin, carbomycin, and spiramycin inhibit protein synthesis by stimulating the dissociation of peptidyl-tRNA from ribosomes.红霉素、碳霉素和螺旋霉素通过刺激肽基 - tRNA从核糖体上解离来抑制蛋白质合成。
Antimicrob Agents Chemother. 1982 May;21(5):811-8. doi: 10.1128/AAC.21.5.811.

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