Montague W, Parkin E N
Horm Metab Res Suppl. 1980;Suppl 10:153-7.
The effect of insulin secretion on the turnover of phospholipids in the pancreatic beta-cell was determined by following the fate, during secretion, of phospholipids prelabelled with 3H-glycerol. The secretion of insulin in response to glucose (20mM) plus isobutyl-methylxanthine (0.5mM) was associated with a calcium dependent increase in the turnover of the major classes of phospholipids and an increase production of 1:2 diacylglycerol and triacylglycerol. In addition, changes in the fatty acid composition of the beta-cell, during insulin secretion, were determined. There was an increase in the proportion of unsaturated fatty acids relative to saturated fatty acids during stimulation by glucose (20mM) plus isobutylmethylxanthine (0.5mM). The results of this study suggest that there are marked changes in the phospholipid composition of the beta-cell during insulin secretion. The regulation of such changes may play an important role in controlling the rate of insulin secretion.
通过追踪在分泌过程中预先用³H - 甘油标记的磷脂的去向，确定胰岛素分泌对胰腺β细胞中磷脂周转的影响。响应葡萄糖（20mM）加异丁基甲基黄嘌呤（0.5mM）的胰岛素分泌与主要磷脂类别的周转的钙依赖性增加以及1:2二酰基甘油和三酰基甘油产量的增加有关。此外，还测定了胰岛素分泌期间β细胞脂肪酸组成的变化。在葡萄糖（20mM）加异丁基甲基黄嘌呤（0.5mM）刺激期间，不饱和脂肪酸相对于饱和脂肪酸的比例增加。这项研究的结果表明，胰岛素分泌期间β细胞的磷脂组成有显著变化。这种变化的调节可能在控制胰岛素分泌速率中起重要作用。
