Ross G D
J Immunol Methods. 1980;37(3-4):197-211. doi: 10.1016/0022-1759(80)90307-5.
The specificity, distribution, and structure of 8 different types of leukocytes membrane complement (C) receptors (CR1, CR2, CR3, and receptors for C1q, beta 1H, C3e, C3a, and C5a) are discussed. Recent data are reviewed on the synthesis of C components by macrophages and B lymphocytes, and how these components may function in the activation of these two cell type by the C system. Commonly used C receptor assay procedures are evaluated in terms of both specificity and sensitivity. Specific assay procedures are recommended for measuring CR1 (C4b-C3b receptor), CR2 (C3d receptor), CR3 (C3bi receptor), and the beta 1H receptor. Assays include both rosette and fluorescence procedures for detection of C receptors on either mouse or human leukocytes. Primary systems have been selected for optimal sensitivity and specificity, and where possible, acceptable alternative systems that are less sensitive or specific are suggested for laboratories lacking facilities for C purification.
本文讨论了8种不同类型白细胞膜补体（C）受体（CR1、CR2、CR3以及C1q、β1H、C3e、C3a和C5a的受体）的特异性、分布和结构。综述了巨噬细胞和B淋巴细胞合成补体成分的最新数据，以及这些成分如何在补体系统激活这两种细胞类型中发挥作用。从特异性和敏感性两方面对常用的补体受体检测方法进行了评估。推荐了用于检测CR1（C4b - C3b受体）、CR2（C3d受体）、CR3（C3bi受体）和β1H受体的特异性检测方法。检测方法包括用于检测小鼠或人白细胞上补体受体的玫瑰花结法和荧光法。已选择了具有最佳敏感性和特异性的主要检测系统，并在可能的情况下，为缺乏补体纯化设施的实验室推荐了敏感性或特异性稍差但可接受的替代系统。
