Odds F C, Abbott A B
Sabouraudia. 1980 Dec;18(4):301-17.
A system of 10 agar plate tests allowed presumptive identification of Candida albicans and differentiation of up to 512 strain types within the species. The yeast isolates were tested for acid and salt tolerance, proteinase production, resistance to 5-fluorocytosine and safranine, and assimilation of urea, sorbose, citrate and glycine. Media were inoculated semiquantitatively with a multiple-pronged device so that 55 yeasts and 5 reference strains could be printed on each plate at once. The results for the 9 strain differentiation tests were arranged in 3 groups to allow simple designation of types by 3-digit numbers. Mouth and vaginal samples from 85 patients and healthy volunteers yielded to 45 different strain types, of which types 153, 157 and 357 were the most prevalent. The reproducibility of the system was good, if attention was paid to the precision which the media were prepared and inoculated. The effect of test variables, including incubation temperature, medium pH and inoculum size, was assessed. Work is presently in progress to extend the system for presumptive identification of other clinically important Candida spp. and differentiation of their strain types.
一个包含10个琼脂平板试验的系统能够对白色念珠菌进行初步鉴定，并区分该菌种内多达512种菌株类型。对酵母分离株进行了耐酸和耐盐性、蛋白酶产生情况、对5-氟胞嘧啶和番红的抗性以及尿素、山梨糖、柠檬酸盐和甘氨酸同化作用的测试。使用多叉接种装置对培养基进行半定量接种，以便在每个平板上一次性接种55个酵母和5个参考菌株。9个菌株鉴别试验的结果分为3组，以便用三位数简单地指定菌株类型。来自85名患者和健康志愿者的口腔及阴道样本产生了45种不同的菌株类型，其中153型、157型和357型最为常见。如果注意培养基制备和接种的精度，该系统的重现性良好。评估了包括培养温度、培养基pH值和接种量在内的测试变量的影响。目前正在开展工作，以扩展该系统用于其他临床重要念珠菌属菌种的初步鉴定及其菌株类型的区分。
