Fine N, Harell A, Binderman I, Landshman N
Stain Technol. 1980 May;55(3):123-7. doi: 10.3109/10520298009067227.
A simple and rapid method is described for processing histological preparations from multilayer cultures growing in plastic Petri dishes, A covering collodion film is utilized to remove the tissue from the plastic dish and transfer it onto a paper block prior to embedding in Paraplast. To avoid any disruption by the collodion of the plasticware, the cultured tissue is first immersed in a solution of collodion and absolute alcohol (1:1) and then covered with pure collodion. All steps are carried out in the cold. This procedure allows morphological, histochemical, immunofluorescent, and autoradiographic studies to be carried out on serial sections of cultured tissue.
本文描述了一种简单快速的方法，用于处理在塑料培养皿中生长的多层培养物的组织学标本。在将组织包埋于石蜡之前，利用一层火棉胶膜将组织从塑料培养皿中取出并转移到纸块上。为避免火棉胶对塑料制品造成任何破坏，先将培养的组织浸入火棉胶与无水乙醇（1:1）的溶液中，然后覆盖纯火棉胶。所有步骤均在低温下进行。该方法可对培养组织的连续切片进行形态学、组织化学、免疫荧光和放射自显影研究。
